p-p70 S6 kinase/S6K1 Antibody (A-6): sc-8416

p-p70 S6 kinase α Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects p70 S6 kinase α phosphorylated at Ser 434 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-p70 S6 kinase α antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p70 S6 kinase α plays a crucial role in regulating protein synthesis and cell growth by phosphorylating ribosomal protein S6, which is essential for translating mRNAs that encode proteins involved in cell cycle progression and growth. This phosphorylation responds to growth factors and nutrient availability, linking extracellular signals to intracellular growth pathways. Activation of p70 S6 kinase α is regulated by upstream signaling pathways, including ERK MAP kinase pathway, underscoring its significance in cellular responses to environmental cues. Understanding p70 S6 kinase α function and regulation is vital for elucidating mechanisms of cell growth and proliferation, making p70 S6 kinase α (A-6) antibody an invaluable research tool for studying these critical biological processes.

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p-p70 S6 kinase/S6K1 Antibody (A-6) References:

1. Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase.  |  Belham, C., et al. 2001. Curr Biol. 11: 1155-67. PMID: 11516946
2. Distinct regulatory mechanism for p70 S6 kinase beta from that for p70 S6 kinase alpha.  |  Minami, T., et al. 2001. Genes Cells. 6: 1003-15. PMID: 11733037
3. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
4. Nuclear localization and regulation of erk- and rsk-encoded protein kinases.  |  Chen, RH., et al. 1992. Mol Cell Biol. 12: 915-27. PMID: 1545823
5. Cloning of the mitogen-activated S6 kinase from rat liver reveals an enzyme of the second messenger subfamily.  |  Kozma, SC., et al. 1990. Proc Natl Acad Sci U S A. 87: 7365-9. PMID: 1699226
6. Structure, expression, and regulation of protein kinases involved in the phosphorylation of ribosomal protein S6.  |  Erikson, RL. 1991. J Biol Chem. 266: 6007-10. PMID: 2007561
7. Protein kinase cascades in meiotic and mitotic cell cycle control.  |  Pelech, SL., et al. 1990. Biochem Cell Biol. 68: 1297-330. PMID: 2085430
8. Molecular structure of a major insulin/mitogen-activated 70-kDa S6 protein kinase.  |  Banerjee, P., et al. 1990. Proc Natl Acad Sci U S A. 87: 8550-4. PMID: 2236064
9. Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.  |  Sweet, LJ., et al. 1990. Mol Cell Biol. 10: 2413-7. PMID: 2325657
10. Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.  |  Alcorta, DA., et al. 1989. Mol Cell Biol. 9: 3850-9. PMID: 2779569

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-p70 S6 kinase/S6K1 Antibody (A-6) has 111 citations in a variety of scientific publications.

Primary antibodies like Anti-p-p70 S6 kinase/S6K1 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-p70 S6 kinase/S6K1 Antibody (A-6) Specifications

Alternate Product Name: p70S6K

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 434

Epitope Target: p70 S6 kinase α

Epitope Species: human

Product Reactivity/Detection: p70 S6 kinase α phosphorylated at Ser 434

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 111 citations in various scientific publications

Available Conjugates/Forms for p-p70 S6 kinase/S6K1 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p70 S6 kinase/S6K1 antibody is p70 S6 kinase/S6K1 Antibody (H-9): sc-8418.