p-p53 Antibody (FP3.2): sc-51690

p-p53 Antibody (FP3.2) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-p53, specifically Ser 392 phosphorylated p53, in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-p53 monoclonal antibody (FP3.2) is offered in a non-conjugated form, allowing for versatile experimental setups. p53 plays a critical role as a tumor suppressor, primarily functioning as a transcription factor that regulates the expression of genes involved in cell cycle control, apoptosis, and DNA repair in response to cellular stress. p53′s ability to localize to the nucleus is essential for function, as p53 binds to specific DNA sequences to activate target genes that promote growth arrest and programmed cell death. Notably, p53 undergoes various post-translational modifications, including phosphorylation, which can significantly influence activity and stability. Phosphorylation at Ser 392 enhances p53′s transcriptional activity and interaction with other regulatory proteins, thereby amplifying tumor-suppressive functions. Dysregulation of p53, often through mutations or aberrant modifications, is a common feature in many cancers, underscoring the importance of studying p53 for understanding tumor biology and developing targeted therapies. anti-p-p53 antibody (FP3.2) provides a valuable tool for studying the functional dynamics and regulatory mechanisms of p53 phosphorylation in both normal cellular processes and cancer research.

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p-p53 Antibody (FP3.2) References:

1. Effect of Boschniakia rossica on expression of GST-P, p53 and p21(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats.  |  Yin, ZZ., et al. 2000. World J Gastroenterol. 6: 812-818. PMID: 11819701
2. Bcl-2 up-regulation and P-p53 down-regulation account for the low sensitivity of murine L929 fibrosarcoma cells to oridonin-induced apoptosis.  |  Huang, J., et al. 2005. Biol Pharm Bull. 28: 2068-74. PMID: 16272691
3. USP10 regulates p53 localization and stability by deubiquitinating p53.  |  Yuan, J., et al. 2010. Cell. 140: 384-96. PMID: 20096447
4. Dimethylfumarate inhibits MIF-induced proliferation of keratinocytes by inhibiting MSK1 and RSK1 activation and by inducing nuclear p-c-Jun (S63) and p-p53 (S15) expression.  |  Gesser, B., et al. 2011. Inflamm Res. 60: 643-53. PMID: 21340650
5. ATM mediates interdependent activation of p53 and ERK through formation of a ternary complex with p-p53 and p-ERK in response to DNA damage.  |  Heo, JI., et al. 2012. Mol Biol Rep. 39: 8007-14. PMID: 22576881
6. p53 and Meduloblastoma.  |  Ramaswamy, V., et al. 2015. Cold Spring Harb Perspect Med. 6: a026278. PMID: 26684332
7. Autophagy and p53.  |  White, E. 2016. Cold Spring Harb Perspect Med. 6: a026120. PMID: 27037419
8. The ATM kinase inhibitor KU-55933 provides neuroprotection against hydrogen peroxide-induced cell damage via a γH2AX/p-p53/caspase-3-independent mechanism: Inhibition of calpain and cathepsin D.  |  Chwastek, J., et al. 2017. Int J Biochem Cell Biol. 87: 38-53. PMID: 28341201
9. Activated mitochondrial apoptosis in hESCs after dissociation involving the PKA/p-p53/Bax signaling pathway.  |  Zhang, L., et al. 2018. Exp Cell Res. 369: 226-233. PMID: 29802837
10. Nuclear HKII-P-p53 (Ser15) Interaction is a Prognostic Biomarker for Chemoresponsiveness and Glycolytic Regulation in Epithelial Ovarian Cancer.  |  Han, CY., et al. 2021. Cancers (Basel). 13: PMID: 34298618

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-p53 Antibody (FP3.2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-p-p53 Antibody (FP3.2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-p53 Antibody (FP3.2) Specifications

Alternate Product Name: TP53

Product Clone Name: FP3.2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 392

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: Ser 392 phosphorylated p53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for p-p53 Antibody (FP3.2): N/A

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.