p-p38 Antibody (D-8) recommended for detection of Tyr 182 phosphorylated p38α, p38β and p38γ of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

p-p38 Antibody (D-8): sc-7973

p-p38 Antibody (D-8) is rated 4.4 out of 5 by 14.
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Datasheets

    • p-p38 Antibody (D-8) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • recommended for detection of Tyr 182 phosphorylated p38α, p38β and p38γ of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • available conjugated to agarose for IP; and to FITC for IF, IHC(P) and FCM
    • also available conjugated to TRITC for IF, IHC(P) and FCM
    • blocking peptide, sc-7973 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p-p38 (E-1): sc-166182 for p-p38 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-p38 (D-8): sc-7973.
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Should I use the secondary mouse antibody which detects IgM ?

Asked by: Susan_SJ
Thank you for your question. Yes, this p-p38 antibody is a mouse IgM and we would recommend using an anti-mouse IgM secondary antibody for detection. Alternatively, you could use our mouse IgG kappa binding protein (m-IgGκ BP), for example, sc-516102. This secondary binds specifically to the kappa light chain of mouse IgG and mouse IgM. A link to the product information on our website is below as a reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-06-26

I have detected p-p38 with this Ab (#7973), but there are two bands at 50 kDa & 25 kDa, not 38~40 kDa. Which band is p-p38? (you can see my band: https://www.researchgate.net/post/Is_there_any_one_who_detected_p-p38_protein_in_western_blot)

Asked by: Susan_SJ
Thank you for your question. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we would be happy to look into this further with you. We want to make sure you're successful in your experiment!
Answered by: Tech Service
Date published: 2017-06-16

Can I use this mouse monoclonal antibody for IF or IHC staining of mouse tissue?

Asked by: Anna99
Yes. Staining on mouse tissue can be done by using a directly conjugated antibody. p-p38 Antibody (D-8): sc-7973 is available conjugated to fluoroscein (sc-7973 FITC), 200 µg/ml, and TRITC (sc-7973 TRITC, 200 µg/ml) for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2017-01-05
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Rated 1 out of 5 by from Not working I can't detect any band at 1:500 dilution overnight in 5%skim milk. Whole cell lysate, CMT93 cell line. Any advice ?
Date published: 2018-12-11
Rated 5 out of 5 by from Worked great The antibody can detect a specific band, at 1:1000. Happy with the antibody.
Date published: 2017-05-20
Rated 5 out of 5 by from clear for WB It was used to detect p-p38 , Bands were clear for WB .
Date published: 2017-03-18
Rated 5 out of 5 by from Broad reactivity Great for multiple species and a range of p38 proteins including alpha, beta and gamma.
Date published: 2017-01-11
Rated 5 out of 5 by from Western blotting Sample:Mouse Cell lysate - whole cell (hepatocytes) Dilution:1/500 Western blotting Conclusion:Strong band
Date published: 2016-12-18
Rated 5 out of 5 by from This has worked very well in WB with SH This has worked very well in WB with SH-SY5Y cell.
Date published: 2015-11-30
Rated 4 out of 5 by from This has worked well in WB This has worked well in WB.
Date published: 2015-11-10
Rated 5 out of 5 by from worked worked
Date published: 2015-05-28
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