Date published: 2026-1-10

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p-eNOS Antibody (pS633.37): sc-136198

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Datasheets
  • p-eNOS Antibody (pS633.37) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against a short amino acid sequence containing phosphorylated Ser 633 of NOS3 of human origin
  • recommended for detection of Ser 633 phosphorylated NOS3 of human origin by WBIP
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for p-eNOS Antibody (pS633.37) for WB applications. This reagent is now offered in a bundle with p-eNOS Antibody (pS633.37) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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    p-NOS3 Antibody (pS633.37) is a mouse monoclonal IgG1 antibody that detects nitric oxide synthase 3 (NOS3) in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). anti-NOS3 antibody (pS633.37) specifically recognizes the phosphorylated form of NOS3 at serine 1177, a critical modification that enhances enzyme activity and promotes nitric oxide (NO) production, a vital signaling molecule in cardiovascular health. NOS3 (pS633.37) monoclonal antibody recognizes a phosphorylation essential for regulating vascular tone, blood pressure, and endothelial function, making NOS3 a key player in maintaining cardiovascular homeostasis. NOS3 is primarily located in endothelial cells lining blood vessels, where activation occurs in response to various stimuli, including insulin-like growth factor-1 and vascular endothelial growth factor. This localization allows for rapid response to changes in blood flow and oxygen levels, facilitating processes such as angiogenesis and vascular remodeling. Using NOS3 (pS633.37) antibody helps researchers gain insights into regulatory mechanisms of endothelial function and cardiovascular disease pathophysiology, ultimately contributing to targeted therapy development.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    p-eNOS Antibody (pS633.37)

    sc-136198
    200 µg/ml
    $322.00

    p-eNOS Antibody (pS633.37): m-IgGκ BP-HRP Bundle

    sc-521342
    200 µg Ab, 40 µg BP
    $361.00

    Hi, I would like to know if this antibody have been tested for bovine samples?  Thank you very much

    Asked by: Anonymous
    Thank you for your question. Anti-p-NOS3 Antibody (pS633.37): sc-136198 is only recommended for detection of Ser 633 phosphorylated NOS3 of human origin by WB IP. We do not have any data that would indicate compatibility with bovine samples.
    Answered by: Tech Support Europe
    Date published: 2021-12-22

    How much sample do you recommend loading on the gel for Western blot detection using p-NOS3 (pS633.37): sc-136198?

    Asked by: Randy McDonald
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-27
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    Rated 4 out of 5 by from Good results by Western blot analysis ofGood results by Western blot analysis of NOS3 phosphorylation in human endothelial cells either untreated (A) or treated (B) with lambda phosphatase.
    Date published: 2014-04-14
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    p-eNOS Antibody (pS633.37) is rated 4.0 out of 5 by 1.
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