p-eNOS Antibody (15E2): sc-81510

p-NOS3 Antibody (15E2) is a mouse monoclonal IgG1 antibody that detects Ser 1177 phosphorylated NOS3 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP). anti-p-NOS3 antibody (15E2) is available in a non-conjugated form, providing flexibility for various experimental setups. NOS3, also known as endothelial nitric oxide synthase, plays a crucial role in cardiovascular health by synthesizing nitric oxide (NO), a key signaling molecule that regulates vascular tone, blood pressure, and angiogenesis. Proper functioning of NOS3 helps maintain cardiovascular homeostasis; dysregulation can lead to various cardiovascular diseases. NOS3 is activated through phosphorylation at Serine 1177, a process mediated by the serine/threonine protein kinase Akt/PKB, which is a significant downstream target of phosphatidylinositol 3-kinase (PI 3-kinase). This phosphorylation event is essential for endothelial cell migration in response to growth factors such as vascular endothelial growth factor (VEGF), highlighting NOS3′s importance in angiogenesis and tissue repair. p-NOS3 (15E2) monoclonal antibody enables researchers to effectively study NOS3 activation state and its implications in various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-eNOS Antibody (15E2) References:

1. Activation of nitric oxide synthase (NOS3) by mechanical activity alters contractile activity in a Ca2+-independent manner in cardiac myocytes: role of troponin I phosphorylation.  |  Kaye, DM., et al. 1999. Biochem Biophys Res Commun. 256: 398-403. PMID: 10079196
2. Akt activates NOS3 and separately restores barrier integrity in H2O2-stressed human cardiac microvascular endothelium.  |  Dossumbekova, A., et al. 2008. Am J Physiol Heart Circ Physiol. 295: H2417-26. PMID: 18931031
3. Akt1 mediates purinergic-dependent NOS3 activation in thick ascending limbs.  |  Silva, GB. and Garvin, JL. 2009. Am J Physiol Renal Physiol. 297: F646-52. PMID: 19570880
4. Angiotensin II stimulates thick ascending limb NO production via AT(2) receptors and Akt1-dependent nitric-oxide synthase 3 (NOS3) activation.  |  Herrera, M. and Garvin, JL. 2010. J Biol Chem. 285: 14932-14940. PMID: 20299462
5. Genetic deletion of NOS3 increases lethal cardiac dysfunction following mouse cardiac arrest.  |  Beiser, DG., et al. 2011. Resuscitation. 82: 115-21. PMID: 20951489
6. Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity.  |  Gebska, MA., et al. 2011. Cardiovasc Res. 90: 353-63. PMID: 21421555
7. Sensitivity to anti-Fas is independent of increased cathepsin D activity and adrenodoxin reductase expression occurring in NOS-3 overexpressing HepG2 cells.  |  Linares, CI., et al. 2015. Biochim Biophys Acta. 1853: 1182-94. PMID: 25712867
8. Plasma gelsolin improves lung host defense against pneumonia by enhancing macrophage NOS3 function.  |  Yang, Z., et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L11-6. PMID: 25957291
9. Cannabidiol Overcomes Oxaliplatin Resistance by Enhancing NOS3- and SOD2-Induced Autophagy in Human Colorectal Cancer Cells.  |  Jeong, S., et al. 2019. Cancers (Basel). 11: PMID: 31195721
10. MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites.  |  Solone, XKV., et al. 2022. FEBS Open Bio. 12: 1075-1086. PMID: 35182051

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-eNOS Antibody (15E2) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-p-eNOS Antibody (15E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-eNOS Antibody (15E2) Specifications

Alternate Product Name: NOS3

Product Clone Name: 15E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: Ser 1177

Epitope Target: NOS3

Epitope Species: human

Product Reactivity/Detection: Ser 1177 phosphorylated NOS3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for p-eNOS Antibody (15E2): N/A

The preferred eNOS antibody is eNOS Antibody (A-9): sc-376751.