p-RELA/NFκB p65 Antibody (27.Ser 536): sc-136548

p-NFκB p65 Antibody (27.Ser 536) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphorylated NFκB p65 in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-NFκB p65 monoclonal antibody (27.Ser 536) is available in conjugated and non-conjugated forms for various experimental applications. NFκB p65 plays a crucial role in regulating immune responses, cell proliferation, and survival, making NFκB p65 a key player in inflammatory processes and cancer development. NFκB p65 activation is tightly controlled, as aberrant signaling can lead to chronic inflammation and tumorigenesis. NFκB p65 primarily resides in the cytoplasm in an inactive form, bound to an inhibitor, and upon stimulation by growth factors or cytokines, NFκB p65 translocates to the nucleus where NFκB p65 binds to specific DNA sequences to initiate transcription of target genes. This dynamic localization enables timely regulation of immune responses and cellular functions, highlighting NFκB p65′s importance in various biological contexts. NFκB p65 undergoes post-translational modifications, including phosphorylation at Serine-536, which enhances transcriptional activity and serves as a critical step in the activation signaling cascade. Understanding these modifications provides insights into mechanisms of diseases associated with dysregulated NFκB signaling.

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p-RELA/NFκB p65 Antibody (27.Ser 536) References:

1. Transcriptional activation of the NF-kappaB p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1).  |  Vermeulen, L., et al. 2003. EMBO J. 22: 1313-24. PMID: 12628924
2. Essential role of RelA Ser311 phosphorylation by zetaPKC in NF-kappaB transcriptional activation.  |  Duran, A., et al. 2003. EMBO J. 22: 3910-8. PMID: 12881425
3. The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.  |  Hatada, EN., et al. 1992. Proc Natl Acad Sci U S A. 89: 2489-93. PMID: 1532257
4. Distinct combinations of NF-kappa B subunits determine the specificity of transcriptional activation.  |  Perkins, ND., et al. 1992. Proc Natl Acad Sci U S A. 89: 1529-33. PMID: 1542644
5. The 65-kDa subunit of human NF-kappa B functions as a potent transcriptional activator and a target for v-Rel-mediated repression.  |  Ballard, DW., et al. 1992. Proc Natl Acad Sci U S A. 89: 1875-9. PMID: 1542686
6. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
7. Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.  |  Meyer, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 966-70. PMID: 1992489
8. Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NFκB P65 Subunit in Spinal Cord Injury.  |  Wang, L., et al. 2018. Cell Physiol Biochem. 50: 1535-1559. PMID: 30376671
9. ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.  |  Lai, X., et al. 2021. J Hazard Mater. 410: 124566. PMID: 33323305
10. Tumor Inflammatory Microenvironment of the Thyroid Cancer: Relationship between Regulatory T-Cell Imbalance, and p-NFΚB (p65) Expression-A Preliminary Study.  |  Pasquali, D., et al. 2023. J Clin Med. 12: PMID: 37959281

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-RELA/NFκB p65 Antibody (27.Ser 536) has 311 citations in a variety of scientific publications.

Primary antibodies like Anti-p-RELA/NFκB p65 Antibody (27.Ser 536) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-RELA/NFκB p65 Antibody (27.Ser 536) Specifications

Alternate Product Name: RELA, RelA, p65, NFKB3

Product Clone Name: 27.Ser 536

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 536

Epitope Target: NFκB p65

Epitope Species: human

Product Reactivity/Detection: Ser 536 phosphorylated NFκB p65

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 311 citations in various scientific publications

Available Conjugates/Forms for p-RELA/NFκB p65 Antibody (27.Ser 536): AC: Agarose, HRP: Horseradish Peroxidase, X: TransCruz Reagent

The preferred RELA/NFκB p65 antibody is RELA/NFκB p65 Antibody (F-6): sc-8008.