p-NFATc4 Antibody (80.S168/170): sc-135771

p-NFATc4 Antibody (80.S168/170) is a mouse monoclonal IgG2a antibody that recognizes dually phosphorylated NFATc4 at Ser 168 and Ser 170 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-NFATc4 (80.S168/170) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. NFATc4, or nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, functions as a crucial transcription factor in regulating immune responses, particularly in T cells. NFATc4 drives inducible expression of cytokine genes, including interleukin-2 (IL-2) and interleukin-4 (IL-4), which are vital for T cell activation and differentiation. Phosphorylation of NFATc4 at specific serine residues, such as Serine-168 and Serine-170, by p38 MAP kinase enables activation and subsequent translocation to the nucleus, where NFATc4 influences gene expression. This regulatory mechanism maintains immune homeostasis and responds to various stimuli, making NFATc4 a key player in both adaptive immunity and potential pathological conditions like autoimmune diseases.

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p-NFATc4 Antibody (80.S168/170) References:

1. Requirement of two NFATc4 transactivation domains for CBP potentiation.  |  Yang, T., et al. 2001. J Biol Chem. 276: 39569-76. PMID: 11514544
2. Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases.  |  Yang, TT., et al. 2002. Mol Cell Biol. 22: 3892-904. PMID: 11997522
3. Targeted disruption of NFATc3, but not NFATc4, reveals an intrinsic defect in calcineurin-mediated cardiac hypertrophic growth.  |  Wilkins, BJ., et al. 2002. Mol Cell Biol. 22: 7603-13. PMID: 12370307
4. Neurotrophins and netrins require calcineurin/NFAT signaling to stimulate outgrowth of embryonic axons.  |  Graef, IA., et al. 2003. Cell. 113: 657-70. PMID: 12787506
5. A ternary complex of transcription factors, Nishéd and NFATc4, and co-activator p300 bound to an intronic sequence, intronic regulatory element, is pivotal for the up-regulation of myosin light chain-2v gene in cardiac hypertrophy.  |  Mathew, S., et al. 2004. J Biol Chem. 279: 41018-27. PMID: 15272022
6. Calcineurin/NFAT-induced up-regulation of the Fas ligand/Fas death pathway is involved in methamphetamine-induced neuronal apoptosis.  |  Jayanthi, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 868-73. PMID: 15644446
7. Recruitment of the extracellular signal-regulated kinase/ribosomal S6 kinase signaling pathway to the NFATc4 transcription activation complex.  |  Yang, TT., et al. 2005. Mol Cell Biol. 25: 907-20. PMID: 15657420
8. Regulation of calcineurin through transcriptional induction of the calcineurin A beta promoter in vitro and in vivo.  |  Oka, T., et al. 2005. Mol Cell Biol. 25: 6649-59. PMID: 16024800
9. Calcineurin regulates aldosterone production via dephosphorylation of NFATC4.  |  Berber, M., et al. 2023. JCI Insight. 8: PMID: 37310791
10. NFATc4 Knockout Promotes Neuroprotection and Retinal Ganglion Cell Regeneration After Optic Nerve Injury.  |  Mackiewicz, J., et al. 2024. Mol Neurobiol. 61: 9383-9401. PMID: 38639863

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-NFATc4 Antibody (80.S168/170) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-NFATc4 Antibody (80.S168/170) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-NFATc4 Antibody (80.S168/170) Specifications

Alternate Product Name: NFATc4

Product Clone Name: 80.S168/170

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Ser 168/170

Epitope Target: NFATc4

Epitope Species: human

Product Reactivity/Detection: Ser 168 and Ser 170 dually phosphorylated NFATc4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-NFATc4 Antibody (80.S168/170): AC: Agarose, HRP: Horseradish Peroxidase, X: TransCruz Reagent

The preferred NFATc4 antibody is NFATc4 Antibody (B-2): sc-271597.