Anti-NFATc4 Antibody (B-2) recommended for detection of NFATc4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-NFATc4 Antibody (B-2): sc-271597

Anti-NFATc4 Antibody (B-2) is rated 4.1 out of 5 by 7.
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Datasheets

    • Anti-NFATc4 Antibody (B-2) is a mouse monoclonal IgG2a κ NFATc4 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 125-198 of NFATc4 of human origin
    • Anti-NFATc4 Antibody (B-2) is recommended for detection of NFATc4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-NFATc4 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-271597 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NFATc4 (B-2): sc-271597.
    • m-IgG2a BP-HRP is the preferred secondary detection reagent for NFATc4 Antibody (B-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with NFATc4 Antibody (B-2) (see ordering information below).
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Does NFATC4 (B-2): sc-271597 antibody detect phosphorylated or dephosphrylated or total NFATC4?

Asked by: MRiaz
Thank you for your question. NFATc4 Antibody (B-2): sc-271597 detects total NFATc4, regardless of post-translational modifications.
Answered by: Tech Support Europe
Date published: 2020-12-18

Can NFATc4 (B-2): sc-271597 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, NFATc4 (B-2): sc-271597 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Used in Westerns Used with 1:500 dilution for western boots. Had difficulty obtaining clear band with low background.
Date published: 2018-03-28
Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from Works in WB In western blot, NFATc4 protein could be detected by this antibody.
Date published: 2017-05-31
Rated 5 out of 5 by from perfect It provides a strong signal with minimal background,I think that it is superior alternative to other companies’ product.
Date published: 2017-03-27
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-08
Rated 5 out of 5 by from Good WB antibody in human transfected 293T Good WB antibody in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic staining Superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human oral mucosa tissue. -SCBT QC
Date published: 2015-04-02
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