p-Met Antibody (F-5): sc-377548

p-Met Antibody (F-5) is a mouse monoclonal IgG3 kappa light chain antibody that specifically detects Tyr 1365 phosphorylated Met in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Met (F-5) antibody is offered in a non-conjugated form, allowing for versatile experimental use. The c-Met oncogene, from which p-Met is derived, was initially identified through transfection analysis in NIH/3T3 cells from a human osteogenic sarcoma cell line treated with a chemical carcinogen. Met proto-oncogene encodes a transmembrane receptor-like protein with intrinsic tyrosine kinase activity, which is crucial for cell proliferation, survival, and migration. p-Met (F-5) monoclonal antibody recognizes Met protein primarily located on the cell surface, where Met serves as the receptor for hepatocyte growth factor, a key player in liver regeneration and tissue repair. The interaction between Met and hepatocyte growth factor is vital for mediating cellular responses to injury and promoting tissue regeneration, highlighting Met′s importance in both normal physiology and cancer progression. Aberrant tyrosine phosphorylation of Met has been implicated in the pathogenesis of several cancers, including gastric carcinoma, where phosphorylation enhances receptor activity, further emphasizing Met′s relevance in oncogenic signaling pathways.

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p-Met Antibody (F-5) References:

1. Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product.  |  Bottaro, DP., et al. 1991. Science. 251: 802-4. PMID: 1846706
2. MET and phosphorylated MET as potential biomarkers in lung cancer.  |  Tretiakova, M., et al. 2011. J Environ Pathol Toxicol Oncol. 30: 341-54. PMID: 22181983
3. Mechanism of met oncogene activation.  |  Park, M., et al. 1986. Cell. 45: 895-904. PMID: 2423252
4. Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinoma.  |  Wu, JG., et al. 2014. BMC Res Notes. 7: 6. PMID: 24393368
5. Characterization of the mouse met proto-oncogene.  |  Chan, AM., et al. 1988. Oncogene. 2: 593-9. PMID: 2838789
6. Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts.  |  Cooper, CS., et al. 1986. EMBO J. 5: 2623-8. PMID: 3023053
7. p145, a protein with associated tyrosine kinase activity in a human gastric carcinoma cell line.  |  Giordano, S., et al. 1988. Mol Cell Biol. 8: 3510-7. PMID: 3211149
8. Foretinib Is Effective against Triple-Negative Breast Cancer Cells MDA-MB-231 In Vitro and In Vivo by Down-Regulating p-MET/HGF Signaling.  |  Ji, X., et al. 2023. Int J Mol Sci. 24: PMID: 36614199
9. Molecular cloning of a new transforming gene from a chemically transformed human cell line.  |  Cooper, CS., et al. Nature. 311: 29-33. PMID: 6590967
10. Phosphorylation of serine 985 negatively regulates the hepatocyte growth factor receptor kinase.  |  Gandino, L., et al. 1994. J Biol Chem. 269: 1815-20. PMID: 8294430

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Met Antibody (F-5) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Met Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Met Antibody (F-5) Specifications

Alternate Product Name: c-Met

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Tyr 1365

Epitope Target: Met

Epitope Species: human

Product Reactivity/Detection: Tyr 1365 phosphorylated Met

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for p-Met Antibody (F-5): P: Blocking Peptide

The preferred Met antibody is Met Antibody (B-2): sc-8057.