Met Antibody (B-2) recommended for detection of Met of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

Met Antibody (B-2): sc-8057

Met Antibody (B-2) is rated 4.5 out of 5 by 13.
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Datasheets

    • Anti-Met Antibody (B-2) is a mouse monoclonal IgG1 (kappa light chain) Met antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1359-1379 at the C-terminus of c-Met p140 of mouse origin
    • Anti-Met Antibody (B-2) is recommended for detection of Met of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Met Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-8057 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Met (B-2): sc-8057.

What is the recommended secondary for use with this Met Antibody (B-2) mouse monoclonal antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from So far so good Works very well for IF on mouse kidney cryosections.
Date published: 2019-04-19
Rated 2 out of 5 by from Did not work in mouse liver lysate Did not work in mouse liver lysate, I tried it with 1:500 dilutions
Date published: 2018-04-12
Rated 2 out of 5 by from Not specific The antibody is very strong and clean but it detects a band near 30-kDa in MCF-7 cells.
Date published: 2017-09-11
Rated 5 out of 5 by from specific bands The specific bands were detected by Western blot and 1:500 in mouse kidney tissues.
Date published: 2017-05-24
Rated 5 out of 5 by from WB 结果很不错 用鼠的内皮细胞提取蛋白,条带很特异。背景很干净。 用去石蜡切片免疫荧光效果还不清楚。过段时间再评论。
Date published: 2017-03-09
Rated 5 out of 5 by from Superb !! Outstanding western data; strong signal with low background; also great IFC staining !!
Date published: 2017-01-27
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-12
Rated 5 out of 5 by from Positive detection of the Met protein by Positive detection of the Met protein by WB using LADMAC whole cell lysate. -SCBT QC
Date published: 2015-04-07
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