p-MEF-2 Antibody (B-11): sc-377535

p-MEF-2 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 387 phosphorylated MEF-2C in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-MEF-2 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEF-2C, a member of the MADS box family of transcription factors, plays a crucial role in muscle development by binding to specific DNA sequences in the regulatory regions of muscle-specific genes, thereby influencing their expression. MEF-2C is predominantly found in skeletal and cardiac muscle cells, where MEF-2C activity is modulated by phosphorylation at Ser 387, a modification that enhances transactivation potential. This phosphorylation is facilitated by Big MAP kinase 1 (BMK1) and p38 MAPK, which are activated in response to various stimuli, including serum. MEF-2C interaction with these kinases is essential for cooperative signaling between Smad proteins and MEF-2, highlighting the importance of post-translational modifications in regulating gene expression during muscle differentiation and development.

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p-MEF-2 Antibody (B-11) References:

1. Smad proteins function as co-modulators for MEF2 transcriptional regulatory proteins.  |  Quinn, ZA., et al. 2001. Nucleic Acids Res. 29: 732-42. PMID: 11160896
2. Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.  |  Yu, YT., et al. 1992. Genes Dev. 6: 1783-98. PMID: 1516833
3. Phosphorylation and alternative pre-mRNA splicing converge to regulate myocyte enhancer factor 2C activity.  |  Zhu, B. and Gulick, T. 2004. Mol Cell Biol. 24: 8264-75. PMID: 15340086
4. Myogenin induces the myocyte-specific enhancer binding factor MEF-2 independently of other muscle-specific gene products.  |  Cserjesi, P. and Olson, EN. 1991. Mol Cell Biol. 11: 4854-62. PMID: 1656214
5. The myoD gene family: nodal point during specification of the muscle cell lineage.  |  Weintraub, H., et al. 1991. Science. 251: 761-6. PMID: 1846704
6. Myogenesis and developmental control genes.  |  Emerson, CP. 1990. Curr Opin Cell Biol. 2: 1065-75. PMID: 2099799
7. MyoD family: a paradigm for development?  |  Olson, EN. 1990. Genes Dev. 4: 1454-61. PMID: 2253873
8. A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.  |  Gossett, LA., et al. 1989. Mol Cell Biol. 9: 5022-33. PMID: 2601707
9. Muscle cell differentiation.  |  Rosenthal, N. 1989. Curr Opin Cell Biol. 1: 1094-101. PMID: 2699798
10. Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.  |  Martin, JF., et al. 1993. Proc Natl Acad Sci U S A. 90: 5282-6. PMID: 8506376

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-MEF-2 Antibody (B-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-MEF-2 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-MEF-2 Antibody (B-11) Specifications

Alternate Product Name: MEF-2

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 387

Epitope Target: MEF-2C

Epitope Species: human

Product Reactivity/Detection: Ser 387 phosphorylated MEF-2C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-MEF-2 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred MEF-2C antibody is MEF-2C Antibody (G-5): sc-518152.