p-Lck Antibody (pY505.4): sc-136184

p-Lck Antibody (pY505.4) is a mouse monoclonal IgG1 antibody that detects Lck in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. p-Lck (pY505.4) antibody specifically recognizes the phosphorylated form of Lck at tyrosine 505, a critical regulatory site that modulates the kinase′s activity. Lck, a member of the Src family of protein tyrosine kinases, plays a pivotal role in T cell signaling and activation, making Lck essential for immune responses. The phosphorylation of Lck at Tyr 505 by Csk serves as a negative regulatory mechanism, inhibiting catalytic activity under certain conditions, while phosphorylation at Tyr 394 enhances activity, promoting T cell activation. This dynamic regulation of Lck is crucial for maintaining the balance between T cell activation and inhibition, which is vital for preventing autoimmune diseases and ensuring effective immune responses. Additionally, Lck is predominantly localized in the plasma membrane of T cells, where Lck interacts with various signaling molecules, including the T cell receptor, to initiate downstream signaling cascades. Anti-Lck antibody (pY505.4) specifically detects phosphorylated Lck, providing valuable insights into the signaling pathways involved in T cell activation and the potential dysregulation associated with various lymphomas and other neoplastic diseases.

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p-Lck Antibody (pY505.4) References:

1. Characterization of the in vivo sites of serine phosphorylation on Lck identifying serine 59 as a site of mitotic phosphorylation.  |  Kesavan, KP., et al. 2002. J Biol Chem. 277: 14666-73. PMID: 11847223
2. The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity.  |  Bergman, M., et al. 1992. EMBO J. 11: 2919-24. PMID: 1639064
3. Expression and interactions of the Src family of tyrosine protein kinases in T lymphocytes.  |  Bolen, JB., et al. 1991. Adv Cancer Res. 57: 103-49. PMID: 1950702
4. A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA.  |  Marth, JD., et al. 1985. Cell. 43: 393-404. PMID: 2416464
5. Regulation of pp56lck during T-cell activation: functional implications for the src-like protein tyrosine kinases.  |  Marth, JD., et al. 1987. EMBO J. 6: 2727-34. PMID: 3119327
6. Activation of p56lck by p72syk through physical association and N-terminal tyrosine phosphorylation.  |  Couture, C., et al. 1994. Mol Cell Biol. 14: 5249-58. PMID: 7518561
7. Stimulation of phosphorylation of Tyr394 by hydrogen peroxide reactivates biologically inactive, non-membrane-bound forms of Lck.  |  Yurchak, LK., et al. 1996. J Biol Chem. 271: 12549-54. PMID: 8647864
8. Regulation of the Lck SH2 domain by tyrosine phosphorylation.  |  Couture, C., et al. 1996. J Biol Chem. 271: 24880-4. PMID: 8798764
9. Structural basis for activation of human lymphocyte kinase Lck upon tyrosine phosphorylation.  |  Yamaguchi, H. and Hendrickson, WA. 1996. Nature. 384: 484-9. PMID: 8945479
10. The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505.  |  Hardwick, JS. and Sefton, BM. 1997. J Biol Chem. 272: 25429-32. PMID: 9325251

The preferred Lck antibody is Lck Antibody (3A5): sc-433.