Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific sites Ser 10, and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Are there any issues with using sc-56745: p-Histone H3 (HTA28) mouse monoclonal primary antibody on mouse samples?
Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56745 conjugates.
Rated 5 out of
Expected results by Western blot analysisExpected results by Western blot analysis of Histone H3 phosphorylation in HeLa and Calyculin A-treated HeLa whole cell lysates. - SCBT QC
Date published: 2015-04-06
Rated 3 out of
Satisfactory Western blot analysis of HistoneSatisfactory Western blot analysis of Histone H3 phosphorylation in untreated, calyculin A treated and calyculin A and lambda protein phosphatase treated HeLa whole cell lystates. Some background - SCBT QC
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