p-Histone H3 Antibody (HTA28) recommended for detection of Ser 28 phosphorylated Histone H3 of mouse, rat, human and bovine origin by WB, IP, IF and FCM

p-Histone H3 Antibody (HTA28): sc-56745

p-Histone H3 Antibody (HTA28) is rated 4.0 out of 5 by 2.
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Datasheets

    • p-Histone H3 Antibody (HTA28) is a rat monoclonal IgG2a provided at 200 µg/ml
    • raised against amino acids 23-35 of p-Histone H3 of human origin
    • recommended for detection of Ser 28 phosphorylated Histone H3 of mouse, rat, human and bovine origin by WB, IP, IF and FCM; not recommended for detection of the unphosphorylated epitope
    • See p-Histone H3 (C-2): sc-374669 for p-Histone H3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Are there any issues with using sc-56745: p-Histone H3 (HTA28) mouse monoclonal primary antibody on mouse samples?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56745 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Expected results by Western blot analysis Expected results by Western blot analysis of Histone H3 phosphorylation in HeLa and Calyculin A-treated HeLa whole cell lysates. - SCBT QC
Date published: 2015-04-06
Rated 3 out of 5 by from Satisfactory Western blot analysis of Histone Satisfactory Western blot analysis of Histone H3 phosphorylation in untreated, calyculin A treated and calyculin A and lambda protein phosphatase treated HeLa whole cell lystates. Some background - SCBT QC
Date published: 2015-01-24
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