p-Histone H2A.X Antibody (Ser 139): sc-517348

p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG1 antibody that detects Ser 139 phosphorylated Histone H2A.X (p-H2A.X) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-p-H2A.X antibody (Ser 139) is available in a non-conjugated form, allowing for versatile experimental setups. Histone H2A.X plays a crucial role in cellular response to DNA damage, particularly in recognizing and repairing double-stranded breaks. Upon DNA damage, H2A.X is rapidly phosphorylated at Ser 139, forming γ-H2A.X, which serves as a marker for DNA damage and is essential for recruiting repair proteins to injury sites. This phosphorylation event is critical for nuclear foci formation, where repair factors such as Rad50, Rad51, and BRCA1 are recruited to facilitate repair. γ-H2A.X not only indicates DNA damage but also plays a pivotal role in signaling pathways that activate cellular repair mechanisms, making γ-H2A.X an important target for studies related to genomic stability and cancer research.

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p-Histone H2A.X Antibody (Ser 139) References:

1. Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139.  |  Rogakou, EP., et al. 2000. J Biol Chem. 275: 9390-5. PMID: 10734083
2. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.  |  Paull, TT., et al. Curr Biol. 10: 886-95. PMID: 10959836
3. Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress.  |  Ward, IM. and Chen, J. 2001. J Biol Chem. 276: 47759-62. PMID: 11673449
4. AID is required to initiate Nbs1/gamma-H2AX focus formation and mutations at sites of class switching.  |  Petersen, S., et al. 2001. Nature. 414: 660-665. PMID: 11740565
5. Histone phosphorylation: a chromatin modification involved in diverse nuclear events.  |  Rossetto, D., et al. 2012. Epigenetics. 7: 1098-108. PMID: 22948226
6. Phosphorylated histone H2A.x in porcine embryos produced by IVF and somatic cell nuclear transfer.  |  Bohrer, RC., et al. 2013. Reproduction. 146: 325-33. PMID: 23858475
7. Resveratrol induces H3 and H4K16 deacetylation and H2A.X phosphorylation in Toxoplasma gondii.  |  Contreras, SM., et al. 2021. BMC Res Notes. 14: 19. PMID: 33413578
8. Exposure to fluoride exacerbates the cognitive deficit of diabetic patients living in areas with endemic fluorosis, as well as of rats with type 2 diabetes induced by streptozotocin via a mechanism that may involve excessive activation of the poly(ADP ribose) polymerase-1/P53 pathway.  |  Xiang, J., et al. 2024. Sci Total Environ. 912: 169512. PMID: 38145685
9. Structure of the mouse H2A.X gene and physical linkage to the UPS locus on chromosome 9: assignment of the human H2A.X gene to 11q23 by sequence analysis.  |  Porcher, C. and Grandchamp, B. 1995. Genomics. 25: 312-3. PMID: 7774939
10. Characterization of the human histone H2A.X gene. Comparison of its promoter with other H2A gene promoters.  |  Ivanova, VS., et al. 1994. J Biol Chem. 269: 24189-94. PMID: 7929075

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Histone H2A.X Antibody (Ser 139) has 116 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Histone H2A.X Antibody (Ser 139) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Histone H2A.X Antibody (Ser 139) Specifications

Alternate Product Name: Histone H2A

Product Clone Name: Ser 139

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: Ser 139

Epitope Target: Histone H2A.X

Epitope Species: human

Product Reactivity/Detection: Ser 139 phosphorylated Histone H2A.X

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 10% glycerol

Research Citations: 116 citations in various scientific publications

Available Conjugates/Forms for p-Histone H2A.X Antibody (Ser 139): N/A

The preferred Histone H2A.X antibody is Histone H2A.X Antibody (938CT5.1.1): sc-517336.