p-GSK-3α Antibody (E-2): sc-365483

p-GSK-3α Antibody (E-2) is a mouse monoclonal IgM antibody that detects Ser 21 phosphorylated GSK-3α in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-GSK-3α antibody (E-2) is available in a non-conjugated form, allowing for versatile experimental use. Glycogen synthase kinase-3α (GSK-3α) plays a crucial role in various cellular processes, including regulation of metabolic enzymes and transcription factors essential for glycogen synthesis and cell adhesion. GSK-3α is primarily located in the cytoplasm but can translocate to the nucleus, where GSK-3α influences gene expression and cellular responses to growth factors. This localization enables GSK-3α to participate in signaling pathways that regulate cell proliferation and survival. GSK-3α interacts with several key proteins, including cAMP-dependent protein kinase A, which phosphorylates GSK-3α at Ser 21, leading to inactivation and modulation of downstream signaling pathways. GSK-3α phosphorylation state determines activity and influence on various biological processes, including energy metabolism and neuronal development. Understanding GSK-3α localization and interactions is vital for elucidating roles in health and disease, particularly in cancer and neurodegenerative disorders.

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p-GSK-3α Antibody (E-2) References:

1. Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation.  |  Hoeflich, KP., et al. 2000. Nature. 406: 86-90. PMID: 10894547
2. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A.  |  Fang, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 11960-5. PMID: 11035810
3. GSK-3alpha regulates production of Alzheimer's disease amyloid-beta peptides.  |  Phiel, CJ., et al. 2003. Nature. 423: 435-9. PMID: 12761548
4. Glycogen synthase kinase-3: functions in oncogenesis and development.  |  Plyte, SE., et al. 1992. Biochim Biophys Acta. 1114: 147-62. PMID: 1333807
5. Suppression of Virulent Porcine Epidemic Diarrhea Virus Proliferation by the PI3K/Akt/GSK-3α/β Pathway.  |  Kong, N., et al. 2016. PLoS One. 11: e0161508. PMID: 27560518
6. Quercetin induces tongue squamous cell carcinoma cell apoptosis via the JNK activation-regulated ERK/GSK-3α/β-mediated mitochondria-dependent apoptotic signaling pathway.  |  Huang, CF., et al. 2022. Oncol Lett. 23: 78. PMID: 35111247
7. Glycogen synthase kinase-3 beta is a dual specificity kinase differentially regulated by tyrosine and serine/threonine phosphorylation.  |  Wang, QM., et al. 1994. J Biol Chem. 269: 14566-74. PMID: 7514173
8. Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.  |  Stambolic, V. and Woodgett, JR. 1994. Biochem J. 303 (Pt 3): 701-4. PMID: 7980435
9. Tyrosine dephosphorylation of glycogen synthase kinase-3 is involved in its extracellular signal-dependent inactivation.  |  Murai, H., et al. 1996. FEBS Lett. 392: 153-60. PMID: 8772194
10. Further evidence that the inhibition of glycogen synthase kinase-3beta by IGF-1 is mediated by PDK1/PKB-induced phosphorylation of Ser-9 and not by dephosphorylation of Tyr-216.  |  Shaw, M., et al. 1997. FEBS Lett. 416: 307-11. PMID: 9373175
11. Essential role for protein kinase B (PKB) in insulin-induced glycogen synthase kinase 3 inactivation. Characterization of dominant-negative mutant of PKB.  |  van Weeren, PC., et al. 1998. J Biol Chem. 273: 13150-6. PMID: 9582355
12. The activation of glycogen synthase by insulin switches from kinase inhibition to phosphatase activation during adipogenesis in 3T3-L1 cells.  |  Brady, MJ., et al. 1998. J Biol Chem. 273: 14063-6. PMID: 9603900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-GSK-3α Antibody (E-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-GSK-3α Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-GSK-3α Antibody (E-2) Specifications

Alternate Product Name: GSK3

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 21

Epitope Target: GSK-3α

Epitope Species: human

Product Reactivity/Detection: Ser 21 phosphorylated GSK-3α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-GSK-3α Antibody (E-2): P: Blocking Peptide

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.