p-Cofilin 1 Antibody (H-2): sc-271923

p-Cofilin 1 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 3 phosphorylated Cofilin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Cofilin 1 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cofilin, a key actin-binding protein, plays a crucial role in regulating actin cytoskeleton, which is essential for cellular motility, division, and shape maintenance. Phosphorylation of Cofilin at Serine 3, mediated by LIM-kinase 1 (LIMK-1), inhibits actin-depolymerizing activity, thereby stabilizing actin filaments and promoting formation of a dynamic actin network. This regulation is vital for cellular responses to signaling pathways that require rapid cytoskeletal rearrangements, making Cofilin a terminal effector in these cascades. Anti-p-Cofilin 1 antibody (H-2) enables researchers to gain insights into mechanisms of actin dynamics and their implications in various physiological and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Cofilin 1 Antibody (H-2) References:

1. Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.  |  Maekawa, M., et al. 1999. Science. 285: 895-8. PMID: 10436159
2. Cofilin phosphorylation and actin cytoskeletal dynamics regulated by rho- and Cdc42-activated LIM-kinase 2.  |  Sumi, T., et al. 1999. J Cell Biol. 147: 1519-32. PMID: 10613909
3. Phosphorylation of Acanthamoeba actophorin (ADF/cofilin) blocks interaction with actin without a change in atomic structure.  |  Blanchoin, L., et al. 2000. J Mol Biol. 295: 203-11. PMID: 10623520
4. A protein kinase from neutrophils that specifically recognizes Ser-3 in cofilin.  |  Lian, JP., et al. 2000. J Biol Chem. 275: 2869-76. PMID: 10644754
5. Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop.  |  Ohashi, K., et al. 2000. J Biol Chem. 275: 3577-82. PMID: 10652353
6. Up-regulation of cofilin-1 in cell senescence associates with morphological change and p27kip1 -mediated growth delay.  |  Tsai, CH., et al. 2021. Aging Cell. 20: e13288. PMID: 33336885
7. Phosphorylation of Cofilin-1 Enhances Paclitaxel Resistance of Epithelial Ovarian Cancer Cells by Inhibiting Apoptosis.  |  Li, M., et al. 2021. Biomed Environ Sci. 34: 465-477. PMID: 34284854
8. The non-muscle ADF/cofilin-1 controls sarcomeric actin filament integrity and force production in striated muscle laminopathies.  |  Vignier, N., et al. 2021. Cell Rep. 36: 109601. PMID: 34433058
9. Cofilin 1 Is Dynamically Modulated During Postnatal Development and by Visual Input in the Mouse Visual Cortex.  |  Laguardia, A., et al. 2023. Neuroscience. 510: 147-156. PMID: 36470478
10. Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.  |  Yang, N., et al. 1998. Nature. 393: 809-12. PMID: 9655398

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Cofilin 1 Antibody (H-2) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Cofilin 1 Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Cofilin 1 Antibody (H-2) Specifications

Alternate Product Name: Cofilin

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 3

Epitope Target: Cofilin 1

Epitope Species: human

Product Reactivity/Detection: Ser 3 phosphorylated Cofilin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p-Cofilin 1 Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Cofilin antibody is Cofilin Antibody (E-8): sc-376476.