p-CaMKII Antibody (22B1): sc-32289

p-CaMKII Antibody (22B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thr 286 phosphorylated CaMKII in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-CaMKII (22B1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a critical subfamily of serine/threonine kinases, with CaMKII playing a pivotal role in cellular signaling pathways. CaMKII is primarily located in the cytoplasm and is known for involvement in synaptic plasticity, which is essential for learning and memory processes. CaMKII′s ability to translocate to the postsynaptic density upon activation allows modulation of synaptic connection strength, thereby influencing neuronal communication and plasticity. CaMKII undergoes various post-translational modifications, including phosphorylation and autophosphorylation, which enhance activity and stability, making CaMKII a key player in numerous physiological processes. Understanding p-CaMKII dynamics and interactions with other proteins is crucial for elucidating roles in both normal cellular function and disease states, particularly in neurodegenerative disorders and cardiac function.

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p-CaMKII Antibody (22B1) References:

1. Chronic caffeine treatment prevents stress-induced LTP impairment: the critical role of phosphorylated CaMKII and BDNF.  |  Alzoubi, KH., et al. 2013. J Mol Neurosci. 49: 11-20. PMID: 22706686
2. Phosphorylated CaMKII levels increase in rat central nervous system after large-dose intravenous remifentanil.  |  Wang, Q., et al. 2013. Med Sci Monit Basic Res. 19: 118-25. PMID: 23549416
3. Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p-CaMKII/ERK/CREB and ox-CaMKII-Dependent MAPK/NF-κB Pathways.  |  Jiang, H., et al. 2021. Oxid Med Cell Longev. 2021: 6673967. PMID: 34527176
4. Mitochondrial Responses to Sublethal Doxorubicin in H9c2 Cardiomyocytes: The Role of Phosphorylated CaMKII.  |  Priyono, AK., et al. 2024. Yonago Acta Med. 67: 41-51. PMID: 38371275
5. The cardiac sarcoplasmic reticulum phospholamban kinase is a distinct delta-CaM kinase isozyme.  |  Baltas, LG., et al. 1995. FEBS Lett. 373: 71-5. PMID: 7589437
6. Characterization of a Ca2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase.  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
7. Calcium/calmodulin-dependent protein kinase II downregulates both calcineurin and protein kinase C-mediated pathways for cytokine gene transcription in human T cells.  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
8. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase).  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
9. Overexpression of Ca2+/calmodulin-dependent protein kinase II inhibits neurite outgrowth of PC12 cells.  |  Tashima, K., et al. 1996. J Neurochem. 66: 57-64. PMID: 8522989
10. Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T lymphocytes.  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-CaMKII Antibody (22B1) has 64 citations in a variety of scientific publications.

Primary antibodies like Anti-p-CaMKII Antibody (22B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-CaMKII Antibody (22B1) Specifications

Alternate Product Name: CAMK2A

Product Clone Name: 22B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 286

Epitope Target: CaMKII

Epitope Species: rat

Product Reactivity/Detection: Thr 286 phosphorylated CaMKII

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 64 citations in various scientific publications

Available Conjugates/Forms for p-CaMKII Antibody (22B1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin