p-c-Jun Antibody (KM-1): sc-822

p-c-Jun Antibody (KM-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects c-Jun protein phosphorylated on Serine 63 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). p-c-Jun (KM-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. c-Jun protein plays a crucial role in cellular processes, acting as a key component of transcription factor AP-1, which regulates gene expression in response to various stimuli, including growth factors and stress signals. This regulation is vital for processes such as cell proliferation, differentiation, and apoptosis, making c-Jun a significant player in oncogenesis and cellular response to external signals. c-Jun forms heterodimers with Fos proteins to enhance transcriptional activity, allowing for a more robust response to environmental cues. Additionally, phosphorylation of c-Jun at Serine 63 is a critical modification that enhances transcriptional activity, linking to various signaling pathways, including those activated by Ras. Understanding c-Jun dynamics and interactions is essential for elucidating mechanisms underlying many cellular functions and disease states.

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p-c-Jun Antibody (KM-1) References:

1. The t(8;21) fusion protein, AML1/ETO, transforms NIH3T3 cells and activates AP-1.  |  Frank, RC., et al. 1999. Oncogene. 18: 1701-10. PMID: 10208431
2. JNK-dependent phosphorylation of c-Jun on serine 63 mediates nitric oxide-induced apoptosis of neuroblastoma cells.  |  Li, L., et al. 2004. J Biol Chem. 279: 4058-65. PMID: 14617628
3. IP-1: a dominant inhibitor of Fos/Jun whose activity is modulated by phosphorylation.  |  Auwerx, J. and Sassone-Corsi, P. 1991. Cell. 64: 983-93. PMID: 1900458
4. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain.  |  Binétruy, B., et al. 1991. Nature. 351: 122-7. PMID: 1903181
5. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
6. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites.  |  Franza, BR., et al. 1988. Science. 239: 1150-3. PMID: 2964084
7. Chromatin association and DNA binding properties of the c-fos proto-oncogene product.  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
8. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.  |  Angel, P., et al. 1988. Nature. 332: 166-71. PMID: 3347253
9. The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
10. Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos.  |  Distel, RJ., et al. 1987. Cell. 49: 835-44. PMID: 3555845

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-c-Jun Antibody (KM-1) has 590 citations in a variety of scientific publications.

Primary antibodies like Anti-p-c-Jun Antibody (KM-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-c-Jun Antibody (KM-1) Specifications

Alternate Product Name: c-Jun

Product Clone Name: KM-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 63

Epitope Target: p-c-Jun

Epitope Species: human

Product Reactivity/Detection: c-Jun p39 phosphorylated on Serine 63

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 590 citations in various scientific publications

Available Conjugates/Forms for p-c-Jun Antibody (KM-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent