p-Bad Antibody (F-6): sc-271963

p-Bad Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 136 phosphorylated Bad in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Bad (F-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Bad, a pro-apoptotic member of the Bcl-2 family, plays a crucial role in regulating apoptosis, and phosphorylation at serine 136 is essential for mediating cell survival signals from growth factors. This phosphorylation event is necessary for the recruitment of 14-3-3 proteins, which enhance the accessibility of serine 155 to survival-promoting kinases, while also inhibiting the pro-apoptotic function of Bad by disrupting interaction with prosurvival Bcl-2 proteins. The intricate regulation of Bad through phosphorylation at these key sites underscores its importance in cellular responses to stress and survival, making p-Bad (F-6) monoclonal antibody an invaluable tool for researchers studying apoptosis and cell signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Bad Antibody (F-6) References:

1. Versatility of BCR/ABL-expressing leukemic cells in circumventing proapoptotic BAD effects.  |  Salomoni, P., et al. 2000. Blood. 96: 676-84. PMID: 10887134
2. 14-3-3 proteins and survival kinases cooperate to inactivate BAD by BH3 domain phosphorylation.  |  Datta, SR., et al. 2000. Mol Cell. 6: 41-51. PMID: 10949026
3. Protein kinase C theta and epsilon promote T-cell survival by a rsk-dependent phosphorylation and inactivation of BAD.  |  Bertolotto, C., et al. 2000. J Biol Chem. 275: 37246-50. PMID: 10976111
4. Phosphatase inhibition promotes antiapoptotic but not proliferative signaling pathways in erythropoietin-dependent HCD57 cells.  |  Lawson, AE., et al. 2000. Blood. 96: 2084-92. PMID: 10979952
5. Phosphorylation of the pro-apoptotic protein BAD on serine 155, a novel site, contributes to cell survival.  |  Virdee, K., et al. 2000. Curr Biol. 10: 1151-4. PMID: 10996800
6. Cannabinoids down-regulate PI3K/Akt and Erk signalling pathways and activate proapoptotic function of Bad protein.  |  Ellert-Miklaszewska, A., et al. 2005. Cell Signal. 17: 25-37. PMID: 15451022
7. Activation of Bad trafficking is involved in the BCR-mediated apoptosis of immature B cells.  |  Malissein, E., et al. 2006. Apoptosis. 11: 1003-12. PMID: 16763785
8. Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic.  |  Kuroda, J., et al. 2006. Proc Natl Acad Sci U S A. 103: 14907-12. PMID: 16997913
9. Bad expression predicts outcome in patients treated with tamoxifen.  |  Cannings, E., et al. 2007. Breast Cancer Res Treat. 102: 173-9. PMID: 17004114
10. Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies.  |  Kim, H., et al. 2006. Nat Cell Biol. 8: 1348-58. PMID: 17115033

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Bad Antibody (F-6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Bad Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Bad Antibody (F-6) Specifications

Alternate Product Name: Bad

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 136

Epitope Target: Bad

Epitope Species: mouse

Product Reactivity/Detection: Ser 136 phosphorylated Bad

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p-Bad Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Bad antibody is Bad Antibody (C-7): sc-8044.