p-Annexin-2/ANXA2 Antibody (85.Tyr 24): sc-135753

p-Annexin II Antibody (85.Tyr 24) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tyr 24 phosphorylated Annexin II in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-p-Annexin II antibody (85.Tyr 24) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. Annexin II, also known as p36, plays a crucial role in cellular processes, particularly in membrane dynamics and cellular signaling regulation. p-Annexin II (85.Tyr 24) monoclonal antibody recognizes proteins primarily located in the cytoplasm that can translocate to the cell membrane in response to calcium ions, which is vital for mediating cellular adhesion and trafficking. Annexin II′s ability to bind phospholipids in a calcium-dependent manner enables participation in essential processes such as endocytosis and exocytosis, which are critical for maintaining cellular homeostasis and responding to external stimuli. Annexin II exists in both monomeric and heterotetrameric forms, with the latter being a complex with the S-100 protein p11, known as calpactin I. This tetrameric form serves as a substrate for various kinases, including protein kinase C and Src family kinases, which can modulate activity through phosphorylation at specific residues like Tyr 24. The phosphorylation state of Annexin II influences interactions with other proteins and overall function in cellular signaling pathways, making anti-p-Annexin II antibody (85.Tyr 24) an invaluable tool for studying these critical biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Annexin-2/ANXA2 Antibody (85.Tyr 24) References:

1. Modulation of annexin II tetramer by tyrosine phosphorylation.  |  Hubaishy, I., et al. 1995. Biochemistry. 34: 14527-34. PMID: 7578058
2. In vivo and in vitro phosphorylation of annexin II in T cells: potential regulation by annexin V.  |  Dubois, T., et al. 1995. Biochem J. 310 (Pt 1): 243-8. PMID: 7646452
3. Structural evolution of the annexin supergene family.  |  Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
4. Annexin II up-regulates cellular levels of p11 protein by a post-translational mechanisms.  |  Puisieux, A., et al. 1996. Biochem J. 313 (Pt 1): 51-5. PMID: 8546709
5. Calcium-dependent binding of S100C to the N-terminal domain of annexin I.  |  Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
6. Annexin II tetramer: structure and function.  |  Waisman, DM. 1995. Mol Cell Biochem. 149-150: 301-22. PMID: 8569746
7. Dexamethasone induces an increase in intracellular and membrane-associated lipocortin-1 (annexin-1) in rat astrocyte primary cultures.  |  McLeod, JD. and Bolton, C. 1995. Cell Mol Neurobiol. 15: 193-205. PMID: 8590451
8. The concerted regulation of cPLA2, COX2, and lipocortin 1 expression by IL-1beta in A549 cells.  |  Croxtal, JD., et al. 1996. Biochem Biophys Res Commun. 220: 491-5. PMID: 8607793
9. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells.  |  Chasserot-Golaz, S., et al. 1996. J Cell Biol. 133: 1217-36. PMID: 8682860
10. Annexin II is a novel player in insulin signal transduction. Possible association between annexin II phosphorylation and insulin receptor internalization.  |  Biener, Y., et al. 1996. J Biol Chem. 271: 29489-96. PMID: 8910617

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Annexin-2/ANXA2 Antibody (85.Tyr 24) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Annexin-2/ANXA2 Antibody (85.Tyr 24) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Annexin-2/ANXA2 Antibody (85.Tyr 24) Specifications

Alternate Product Name: Annexin II

Product Clone Name: 85.Tyr 24

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 24

Epitope Target: Annexin II

Epitope Species: human

Product Reactivity/Detection: Tyr 24 phosphorylated Annexin II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for p-Annexin-2/ANXA2 Antibody (85.Tyr 24): AC: Agarose, HRP: Horseradish Peroxidase

The preferred Annexin-2/ANXA2 antibody is Annexin-2/ANXA2 Antibody (C-10): sc-28385.