p-αB-crystallin Antibody (F-1): sc-365884

p-αB-crystallin Antibody (F-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects p-αB-crystallin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-αB-crystallin antibody (F-1) is available as the non-conjugated form. p-αB-crystallin protein, also known as phosphorylated heat shock protein beta-5, plays a crucial role in cellular stress responses, particularly in protecting cells from damage caused by heat and other stressors. p-αB-crystallin protein functions as a molecular chaperone, helping to stabilize and refold denatured proteins, thereby preventing aggregation and promoting cell survival under stress conditions. p-αB-crystallin protein′s importance is underscored by involvement in various diseases; elevated levels have been linked to neurodegenerative disorders, and mutations in the encoding gene can lead to desmin-related myopathy. Additionally, phosphorylation of p-αB-crystallin at Ser 59 by p38 MAPK substrate MAPKAPK-2 is a critical post-translational modification that regulates chaperone activity and cellular localization, further highlighting p-αB-crystallin protein′s significance in maintaining cellular homeostasis.

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p-αB-crystallin Antibody (F-1) References:

1. Mutation R120G in alphaB-crystallin, which is linked to a desmin-related myopathy, results in an irregular structure and defective chaperone-like function.  |  Bova, MP., et al. 1999. Proc Natl Acad Sci U S A. 96: 6137-42. PMID: 10339554
2. Differential translocation or phosphorylation of alpha B crystallin cannot be detected in ischemically preconditioned rabbit cardiomyocytes.  |  Armstrong, SC., et al. 2000. J Mol Cell Cardiol. 32: 1301-14. PMID: 10860771
3. alpha-crystallin prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature the stabilized partially denatured protein in an ATP-dependent manner.  |  Wang, K. and Spector, A. 2000. Eur J Biochem. 267: 4705-12. PMID: 10903503
4. Lens crystallins and their microbial homologs: structure, stability, and function.  |  Jaenicke, R. and Slingsby, C. 2001. Crit Rev Biochem Mol Biol. 36: 435-99. PMID: 11724156
5. Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.  |  Narberhaus, F. 2002. Microbiol Mol Biol Rev. 66: 64-93; table of contents. PMID: 11875128
6. α-B Crystallin Reverses High Diastolic Stiffness of Failing Human Cardiomyocytes.  |  Franssen, C., et al. 2017. Circ Heart Fail. 10: e003626. PMID: 28242778
7. Role of the Alpha-B-Crystallin Protein in Cardiomyopathic Disease.  |  Thorkelsson, A. and Chin, MT. 2024. Int J Mol Sci. 25: PMID: 38474073
8. Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors.  |  Neufer, PD. and Benjamin, IJ. 1996. J Biol Chem. 271: 24089-95. PMID: 8798647
9. Autosomal dominant congenital cataract associated with a missense mutation in the human alpha crystallin gene CRYAA.  |  Litt, M., et al. 1998. Hum Mol Genet. 7: 471-4. PMID: 9467006
10. The small heat-shock protein, alphaB-crystallin, has a variable quaternary structure.  |  Haley, DA., et al. 1998. J Mol Biol. 277: 27-35. PMID: 9514758
11. Phosphorylations of alpha A- and alpha B-crystallin.  |  Kantorow, M. and Piatigorsky, J. 1998. Int J Biol Macromol. 22: 307-14. PMID: 9650085
12. Purification of the stress protein alpha B-crystallin and its differentially phosphorylated forms.  |  van Noort, JM., et al. 1998. J Immunol Methods. 221: 159-68. PMID: 9894907

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-αB-crystallin Antibody (F-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-αB-crystallin Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-αB-crystallin Antibody (F-1) Specifications

Alternate Product Name: p-CRYAB

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Ser 59

Epitope Target: αB-crystallin

Epitope Species: human

Product Reactivity/Detection: Ser 59 phosphorylated αB-crystallin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-αB-crystallin Antibody (F-1): P: Blocking Peptide

The preferred αB-crystallin antibody is αB-crystallin Antibody (F-10): sc-137129.