p-ACCα Antibody (F-2): sc-271965

p-ACCα Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-ACCα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-ACCα (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Acetyl-CoA carboxylase (ACC) plays a crucial role in lipid metabolism by catalyzing the conversion of acetyl-CoA to malonyl-CoA, which is the rate-limiting step in fatty acid synthesis. This process is vital for maintaining energy homeostasis and regulating fatty acid levels in cells. p-ACCα, specifically, is essential for the biogenesis of long-chain fatty acids, while ACCβ is involved in controlling mitochondrial fatty acid oxidation. p-ACCα activity is regulated through phosphorylation and dephosphorylation of specific serine residues, which toggle between active and inactive states, as well as through allosteric modulation by metabolites such as citrate and palmitoyl-CoA. ACCα is located on human chromosome 17 and is predominantly expressed in lipogenic tissues, highlighting significance in metabolic pathways. Understanding p-ACCα regulatory mechanisms is critical for elucidating roles in metabolic disorders and developing therapeutic strategies targeting lipid metabolism.

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p-ACCα Antibody (F-2) References:

1. The subcellular localization of acetyl-CoA carboxylase 2.  |  Abu-Elheiga, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 1444-9. PMID: 10677481
2. Exercise diminishes the activity of acetyl-CoA carboxylase in human muscle.  |  Dean, D., et al. 2000. Diabetes. 49: 1295-300. PMID: 10923628
3. Thyroid hormone stimulates acetyl-coA carboxylase-alpha transcription in hepatocytes by modulating the composition of nuclear receptor complexes bound to a thyroid hormone response element.  |  Zhang, Y., et al. 2001. J Biol Chem. 276: 974-83. PMID: 11027684
4. Cloning of human acetyl-CoA carboxylase beta promoter and its regulation by muscle regulatory factors.  |  Lee, JJ., et al. 2001. J Biol Chem. 276: 2576-85. PMID: 11076940
5. Activation of acetyl-CoA carboxylase by a glutamate- and magnesium-sensitive protein phosphatase in the islet beta-cell.  |  Kowluru, A., et al. 2001. Diabetes. 50: 1580-7. PMID: 11423479
6. Developmental regulation of alternatively spliced acetyl-CoA carboxylase-alpha mRNAs encoding isozymes with or without an eight amino acid domain upstream of the Ser-1200 phosphorylation motif in the mammary gland.  |  Barber, MC., et al. 2001. J Mol Endocrinol. 27: 349-56. PMID: 11719287
7. Characterisation of an N-terminal variant of acetyl-CoA carboxylase-alpha: expression in human tissues and evolutionary aspects.  |  Travers, MT., et al. 2003. Biochim Biophys Acta. 1634: 97-106. PMID: 14643797
8. Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition.  |  Sinilnikova, OM., et al. 2007. Cancer Epidemiol Biomarkers Prev. 16: 409-15. PMID: 17372234
9. Up-regulation of acetyl-CoA carboxylase alpha and fatty acid synthase by human epidermal growth factor receptor 2 at the translational level in breast cancer cells.  |  Yoon, S., et al. 2007. J Biol Chem. 282: 26122-31. PMID: 17631500
10. Dual role of insulin-like growth factor-1 in acetyl-CoA carboxylase-alpha activity in human colon cancer cells HCT-8: downregulating its expression and phosphorylation.  |  Luo, DX., et al. 2011. Mol Cell Biochem. 357: 255-62. PMID: 21638027
11. Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles.  |  Raimondo, S., et al. 2018. J Proteomics. 173: 1-11. PMID: 29197582
12. Regulation of mammalian acetyl-coenzyme A carboxylase.  |  Kim, KH. 1997. Annu Rev Nutr. 17: 77-99. PMID: 9240920

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ACCα Antibody (F-2) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ACCα Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ACCα Antibody (F-2) Specifications

Alternate Product Name: p-Acetyl-CoA Carboxylase Alpha

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 78/80

Epitope Target: ACCα

Epitope Species: human

Product Reactivity/Detection: Ser 78 and Ser 80 dually phosphorylated ACCα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for p-ACCα Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ACCα antibody is ACCα Antibody (D-5): sc-137104.