Oxytocin-R Double Nickase Plasmid (h): sc-400641-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Oxytocin-R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Oxytocin-R Double Nickase Plasmid (h) and Oxytocin-R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OXTR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Oxytocin-R Antibody (C-4): sc-515809
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oxytocin-R Double Nickase Plasmid (h) | sc-400641-NIC | 20 µg | $410.00 | |||
Oxytocin-R Double Nickase Plasmid (h2) | sc-400641-NIC-2 | 20 µg | $410.00 |
Human OXTR encodes the oxytocin receptor (Oxytocin-R), a class A GPCR that couples primarily to Gq/11 to stimulate phospholipase C signaling, intracellular Ca2+ mobilization, and PKC-dependent transcriptional responses. OXTR activity intersects with MAPK/ERK and PI3K pathways to regulate smooth muscle contractility, secretory programs, and cell–cell communication, with context-dependent effects across reproductive tissues and the central nervous system. In neurons and glia, OXTR signaling shapes synaptic plasticity and social-behavioral circuits, while in peripheral tissues it modulates inflammatory and stress-responsive programs. Genetic and expression variation in OXTR has been investigated in neuropsychiatric and neurodevelopmental phenotypes, as well as reproductive and metabolic traits, making it a useful target for mechanistic studies of GPCR-mediated signaling.
Oxytocin-R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OXTR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OXTR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OXTR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OXTR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.