Oxytocin-R CRISPR/Cas9 KO Plasmid (h): sc-400641
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Oxytocin-R CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Oxytocin-R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Oxytocin-R Antibody (C-4): sc-515809
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oxytocin-R CRISPR/Cas9 KO Plasmid (h) | sc-400641 | 20 µg | $397.00 |
Overview
OXTR encodes the human oxytocin receptor (Oxytocin-R), a G protein–coupled receptor that primarily couples to Gq/11 to activate phospholipase C, inositol trisphosphate production, and intracellular Ca2+ mobilization, with additional context-dependent signaling through MAPK/ERK and PI3K pathways. Oxytocin-R regulates smooth muscle contractility, neuroendocrine secretion, and synaptic signaling that influence social behavior and stress responses. In immune and stromal contexts, OXTR signaling can modulate cytokine release, cell migration, and tissue remodeling programs. Altered OXTR expression or signaling has been studied in neurodevelopmental and psychiatric phenotypes, reproductive biology disorders, and cancers where GPCR-driven pathways contribute to proliferation and microenvironmental interactions.
Oxytocin-R CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OXTR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OXTR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OXTR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Oxytocin-R protein expression.
This CRISPR knockout system enables efficient generation of OXTR-deficient cell models for investigation of Oxytocin-R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OXTR exon(s) critical for Oxytocin-R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OXTR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Oxytocin-R CRISPR/Cas9 KO Plasmid (h) and Oxytocin-R CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OXTR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Oxytocin-R HDR Plasmid (h) and Oxytocin-R HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OXTR homology arms to support homology-directed repair at defined OXTR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.