OTULIN CRISPR/Cas9 KO Plasmid (m): sc-436617
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTULIN CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OTULIN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTULIN CRISPR/Cas9 KO Plasmid (m) | sc-436617 | 20 µg | $397.00 |
Overview
Otulin encodes OTULIN, a deubiquitinase that selectively hydrolyzes Met1 (linear)-linked ubiquitin chains generated by the LUBAC complex, thereby shaping ubiquitin-dependent signal propagation. In mouse cells, OTULIN functions as a key negative regulator of NF-κB and inflammatory signaling by controlling linear ubiquitination of pathway components such as RIPK1 and NEMO/IKBKG, influencing cytokine responses and cell death decisions. Disruption of OTULIN perturbs proteostasis and immune homeostasis, linking this pathway to autoinflammatory phenotypes and broader inflammatory disease mechanisms. OTULIN also intersects with innate immune signaling and stress responses where linear ubiquitin editing tunes the amplitude and duration of receptor-triggered signaling.
OTULIN CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Otulin gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Otulin together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Otulin open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OTULIN protein expression.
This CRISPR knockout system enables efficient generation of Otulin-deficient cell models for investigation of OTULIN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Otulin exon(s) critical for OTULIN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Otulin genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OTULIN CRISPR/Cas9 KO Plasmid (m) and OTULIN CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Otulin locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OTULIN HDR Plasmid (m) and OTULIN HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Otulin homology arms to support homology-directed repair at defined Otulin target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.