Otoferlin Antibody (C-12): sc-271092

Otoferlin Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Otoferlin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Otoferlin antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Otoferlin is a single-pass type II membrane protein composed of 1,230 amino acid residues and exists in four isoforms, with isoform 1 being the full-length version and isoforms 2-4 representing shorter variants. Expression of isoforms 1 and 3 is primarily found in the adult brain, while isoform 2 is expressed in a variety of tissues, including the fetus, adult brain, heart, placenta, skeletal muscle, and kidney. Notably, Otoferlin contains three C2 domains and a single carboxy-terminal transmembrane domain, which are crucial for calcium-triggered synaptic vesicle-plasma membrane fusion, a process essential for neurotransmitter release. The Otoferlin gene, OTOF, is located at 2p23-p22, with its 5′ region being centromeric, and mutations in this gene have been linked to auditory processing disorders, highlighting Otoferlin′s importance in hearing. Otoferlin (C-12) antibody is an invaluable tool for researchers studying synaptic function and the molecular mechanisms underlying deafness.

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Otoferlin Antibody (C-12) References:

1. A mutation in OTOF, encoding otoferlin, a FER-1-like protein, causes DFNB9, a nonsyndromic form of deafness.  |  Yasunaga, S., et al. 1999. Nat Genet. 21: 363-9. PMID: 10192385
2. Deafness heterogeneity in a Druze isolate from the Middle East: novel OTOF and PDS mutations, low prevalence of GJB2 35delG mutation and indication for a new DFNB locus.  |  Adato, A., et al. 2000. Eur J Hum Genet. 8: 437-42. PMID: 10878664
3. Q829X, a novel mutation in the gene encoding otoferlin (OTOF), is frequently found in Spanish patients with prelingual non-syndromic hearing loss.  |  Migliosi, V., et al. 2002. J Med Genet. 39: 502-6. PMID: 12114484
4. Substitutions in the conserved C2C domain of otoferlin cause DFNB9, a form of nonsyndromic autosomal recessive deafness.  |  Mirghomizadeh, F., et al. 2002. Neurobiol Dis. 10: 157-64. PMID: 12127154
5. Uncommon cytidine-homopolymer dimorphism in 5'-UTR of the human otoferlin gene.  |  Mirghomizadeh, F., et al. 2003. Int J Mol Med. 11: 63-4. PMID: 12469219
6. Auditory neuropathy in patients carrying mutations in the otoferlin gene (OTOF).  |  Rodríguez-Ballesteros, M., et al. 2003. Hum Mutat. 22: 451-6. PMID: 14635104
7. A novel missense mutation in a C2 domain of OTOF results in autosomal recessive auditory neuropathy.  |  Tekin, M., et al. 2005. Am J Med Genet A. 138: 6-10. PMID: 16097006
8. OTOF mutations revealed by genetic analysis of hearing loss families including a potential temperature sensitive auditory neuropathy allele.  |  Varga, R., et al. 2006. J Med Genet. 43: 576-81. PMID: 16371502
9. Transient deafness due to temperature-sensitive auditory neuropathy.  |  Starr, A., et al. 1998. Ear Hear. 19: 169-79. PMID: 9657592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Otoferlin Antibody (C-12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Otoferlin Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Otoferlin Antibody (C-12) Specifications

Alternate Product Name: Otoferlin

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 691-840

Epitope Target: Otoferlin

Epitope Species: human

Product Reactivity/Detection: Otoferlin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Otoferlin Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin