Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
osteocalcin CRISPR/Cas9 KO Plasmid (h): sc-400299
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- osteocalcin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the osteocalcin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: osteocalcin Antibody (G-5): sc-365797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
osteocalcin CRISPR/Cas9 KO Plasmid (h) | sc-400299 | 20 µg | $397.00 |
Overview
BGLAP encodes osteocalcin, a secreted, vitamin K–dependent non-collagenous protein produced by osteoblasts and deposited in the bone extracellular matrix as a key marker of osteogenic differentiation. Osteocalcin binds hydroxyapatite and contributes to mineral maturation and calcium homeostasis, linking bone-forming programs with extracellular matrix organization. Its expression is coordinated with osteoblast regulatory networks, including RUNX2-driven differentiation and signaling pathways that couple bone remodeling to osteoclast–osteoblast communication. Altered BGLAP/osteocalcin regulation is frequently studied in the context of bone turnover phenotypes, skeletal mineralization disorders, and tumor–bone microenvironment interactions.
osteocalcin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BGLAP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BGLAP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BGLAP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish osteocalcin protein expression.
This CRISPR knockout system enables efficient generation of BGLAP-deficient cell models for investigation of osteocalcin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BGLAP exon(s) critical for osteocalcin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BGLAP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by osteocalcin CRISPR/Cas9 KO Plasmid (h) and osteocalcin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BGLAP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by osteocalcin HDR Plasmid (h) and osteocalcin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BGLAP homology arms to support homology-directed repair at defined BGLAP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.