Osteoadherin Antibody (B-10): sc-271102

Osteoadherin Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Osteoadherin of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Osteoadherin antibody (B-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Osteoadherin, also known as OSAD, is a bone proteoglycan that plays a crucial role in the extracellular matrix, facilitating cell adhesion through interaction with integrin αvβ3. OSAD′s structure features eleven B-type, leucine-rich repeats, which contribute to stability and functionality in the extracellular environment. Notably, Osteoadherin contains several post-translational modifications, including four putative sites for tyrosine sulfation and six assumed sites for N-linked glycosylation, which are essential for biological activity and interactions with other proteins. A unique C-terminal domain of Osteoadherin, which is highly acidic, further distinguishes OSAD within the small leucine-rich proteoglycan family and plays a significant role in interactions with other extracellular matrix components. Osteoadherin expression is primarily localized to the extracellular space, underscoring OSAD′s importance in bone biology and tissue integrity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Osteoadherin Antibody (B-10) References:

1. Tissue distribution of a novel cell binding protein, osteoadherin, in the rat.  |  Shen, Z., et al. 1999. Matrix Biol. 18: 533-42. PMID: 10607915
2. Super-motifs and evolution of tandem leucine-rich repeats within the small proteoglycans--biglycan, decorin, lumican, fibromodulin, PRELP, keratocan, osteoadherin, epiphycan, and osteoglycin.  |  Matsushima, N., et al. 2000. Proteins. 38: 210-25. PMID: 10656267
3. Expression of the small leucine-rich proteoglycan osteoadherin/osteomodulin in human dental pulp and developing rat teeth.  |  Buchaille, R., et al. 2000. Bone. 27: 265-70. PMID: 10913920
4. Ultrastructural distribution of osteoadherin in rat bone shows a pattern similar to that of bone sialoprotein.  |  Ramstad, VE., et al. 2003. Calcif Tissue Int. 72: 57-64. PMID: 12384815
5. TGF beta 1 signaling and stimulation of osteoadherin in human odontoblasts in vitro.  |  Lucchini, M., et al. 2002. Connect Tissue Res. 43: 345-53. PMID: 12489179
6. Alpha v beta 3 integrin expression in human odontoblasts and co-localization with osteoadherin.  |  Lucchini, M., et al. 2004. J Dent Res. 83: 552-6. PMID: 15218045
7. Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone.  |  Solberg, LB., et al. 2006. Micron. 37: 347-54. PMID: 16387503
8. Bone matrix proteins: isolation and characterization of a novel cell-binding keratan sulfate proteoglycan (osteoadherin) from bovine bone.  |  Wendel, M., et al. 1998. J Cell Biol. 141: 839-47. PMID: 9566981
9. Osteoadherin, a cell-binding keratan sulfate proteoglycan in bone, belongs to the family of leucine-rich repeat proteins of the extracellular matrix.  |  Sommarin, Y., et al. 1998. J Biol Chem. 273: 16723-9. PMID: 9642227

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Osteoadherin Antibody (B-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Osteoadherin Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Osteoadherin Antibody (B-10) Specifications

Alternate Product Name: Osteoadherin

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 221-380

Epitope Target: Osteoadherin

Epitope Species: human

Product Reactivity/Detection: Osteoadherin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Osteoadherin Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin