ORC2 Antibody (3G6): sc-32734

ORC2 Antibody (3G6) is a rat monoclonal IgG2a antibody that detects ORC2 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-ORC2 antibody (3G6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORC2 is a critical component of the origin recognition complex (ORC), which is essential for the initiation of DNA replication. ORC2 plays a vital role in forming pre-replication complexes necessary for accurate and timely genome replication. ORC2, along with other ORC subunits, binds to specific origins of replication in proliferating cell nuclei, serving as a scaffold for additional initiation factor assembly. While ORC1 and ORC2 are expressed at similar levels throughout the cell cycle, ORC2 uniquely remains stably bound to chromatin, highlighting ORC2′s importance in maintaining replication process integrity. ORC2′s interaction with other ORC proteins, such as ORC3, ORC4, and ORC5, to form a core complex is crucial for G1-S transition in mammalian cells, underscoring ORC2′s role in cell cycle regulation and potential implications in cancer biology.

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ORC2 Antibody (3G6) References:

1. Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.  |  Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
2. Identification and characterization of the human ORC6 homolog.  |  Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
3. Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins.  |  Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
4. Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.  |  Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
5. The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells.  |  Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449
6. Assembly of the human origin recognition complex.  |  Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
7. Architecture of the human origin recognition complex.  |  Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
8. Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance.  |  Prasanth, SG., et al. 2004. EMBO J. 23: 2651-63. PMID: 15215892
9. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability.  |  Huang, C., et al. 2016. Cell Rep. 15: 147-157. PMID: 27052177
10. Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2.  |  Wang, R., et al. 2017. Oncotarget. 8: 70142-70155. PMID: 29050267
11. The ORFIUS complex regulates ORC2 localization at replication origins.  |  Yang, Z., et al. 2024. NAR Cancer. 6: zcae003. PMID: 38288445
12. Identification of HsORC4, a member of the human origin of replication recognition complex.  |  Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ORC2 Antibody (3G6) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-ORC2 Antibody (3G6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ORC2 Antibody (3G6) Specifications

Alternate Product Name: ORC2

Product Clone Name: 3G6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 557-574

Epitope Target: ORC2

Epitope Species: human

Product Reactivity/Detection: ORC2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for ORC2 Antibody (3G6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent