opticin Antibody (F-2): sc-271306
- opticin Antibody (F-2) is a mouse monoclonal IgG2b κ opticin antibody provided at 200 µg/ml
- raised against amino acids 191-275 mapping within an internal region of opticin of human origin
- opticin Antibody (F-2) is recommended for detection of opticin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-opticin Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for opticin Antibody (F-2) for WB applications. These reagents are now offered in bundles with opticin Antibody (F-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
Opticin Antibody (F-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects opticin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-opticin antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Opticin, also known as OPTC or OPT, is a 332 amino acid secreted protein characterized by the presence of seven leucine-rich repeats (LRRs), which are crucial for structural integrity and function. These LRRs form a hydrophobic α/β horseshoe fold, allowing opticin to effectively bind collagen fibrils within the extracellular matrix. This interaction is vital for regulating collagen fibril spacing, morphology, and organization, which are essential for maintaining the structural integrity of tissues, particularly in ocular regions such as the retina, cornea, and iris. Additionally, opticin undergoes post-translational O-glycosylation, a modification that may influence function and has been implicated in the pathogenesis of primary open-angle glaucoma. With diverse applications and critical role in tissue organization, opticin monoclonal antibody (F-2) serves as an invaluable tool for researchers studying extracellular matrix dynamics and related ocular conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
opticin Antibody (F-2) References:
- Identification in vitreous and molecular cloning of opticin, a novel member of the family of leucine-rich repeat proteins of the extracellular matrix. | Reardon, AJ., et al. 2000. J Biol Chem. 275: 2123-9. PMID: 10636917
- Cloning, modeling, and chromosomal localization for a small leucine-rich repeat proteoglycan (SLRP) family member expressed in human eye. | Hobby, P., et al. 2000. Mol Vis. 6: 72-8. PMID: 10837509
- Protein localization in the human eye and genetic screen of opticin. | Friedman, JS., et al. 2002. Hum Mol Genet. 11: 1333-42. PMID: 12019215
- Characterization of opticin and evidence of stable dimerization in solution. | Le Goff, MM., et al. 2003. J Biol Chem. 278: 45280-7. PMID: 12951322
- Immunolocalisation of opticin in the human eye. | Ramesh, S., et al. 2004. Br J Ophthalmol. 88: 697-702. PMID: 15090426
- Evaluation of the OPTC gene in primary open angle glaucoma: functional significance of a silent change. | Acharya, M., et al. 2007. BMC Mol Biol. 8: 21. PMID: 17359525
- Role of CYP1B1, MYOC, OPTN, and OPTC genes in adult-onset primary open-angle glaucoma: predominance of CYP1B1 mutations in Indian patients. | Kumar, A., et al. 2007. Mol Vis. 13: 667-76. PMID: 17563717
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
opticin Antibody (F-2) | sc-271306 | 200 µg/ml | $322.00 | |||
opticin Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-529161 | 200 µg Ab; 10 µg BP | $361.00 | |||
opticin Antibody (F-2): m-IgGκ BP-HRP Bundle | sc-521908 | 200 µg Ab, 40 µg BP | $361.00 | |||
opticin Antibody (F-2): m-IgG2b BP-HRP Bundle | sc-550083 | 200 µg Ab; 10 µg BP | $361.00 | |||
opticin Antibody (F-2) AC | sc-271306 AC | 500 µg/ml, 25% agarose | $424.00 | |||
opticin Antibody (F-2) HRP | sc-271306 HRP | 200 µg/ml | $322.00 | |||
opticin Antibody (F-2) FITC | sc-271306 FITC | 200 µg/ml | $336.00 | |||
opticin Antibody (F-2) PE | sc-271306 PE | 200 µg/ml | $349.00 | |||
opticin Antibody (F-2) Alexa Fluor® 488 | sc-271306 AF488 | 200 µg/ml | $364.00 | |||
opticin Antibody (F-2) Alexa Fluor® 546 | sc-271306 AF546 | 200 µg/ml | $364.00 | |||
opticin Antibody (F-2) Alexa Fluor® 594 | sc-271306 AF594 | 200 µg/ml | $364.00 | |||
opticin Antibody (F-2) Alexa Fluor® 647 | sc-271306 AF647 | 200 µg/ml | $364.00 | |||
opticin Antibody (F-2) Alexa Fluor® 680 | sc-271306 AF680 | 200 µg/ml | $364.00 | |||
opticin Antibody (F-2) Alexa Fluor® 790 | sc-271306 AF790 | 200 µg/ml | $364.00 |