Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
OPA1 Double Nickase Plasmid (h): sc-401555-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OPA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OPA1 Double Nickase Plasmid (h) and OPA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OPA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OPA1 Antibody (D-9): sc-393296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OPA1 Double Nickase Plasmid (h) | sc-401555-NIC | 20 µg | $410.00 | |||
OPA1 Double Nickase Plasmid (h2) | sc-401555-NIC-2 | 20 µg | $410.00 |
OPA1 encodes a dynamin-like GTPase anchored to the inner mitochondrial membrane that regulates mitochondrial inner membrane fusion, cristae architecture, and respiratory chain efficiency. Through coordination with mitochondrial fission–fusion machinery and proteolytic processing by OMA1 and YME1L, OPA1 helps maintain mitochondrial membrane potential and limits stress-induced cytochrome c mobilization. OPA1 dysfunction perturbs oxidative phosphorylation, elevates reactive oxygen species, and alters mitophagy and apoptotic sensitivity. Variants in OPA1 are strongly linked to dominant optic atrophy and broader neurodegenerative phenotypes, making it a key target for mitochondrial dynamics and neuronal bioenergetics research.
OPA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OPA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OPA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OPA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OPA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.