Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Op18 Double Nickase Plasmid (h): sc-401656-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Op18 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Op18 Double Nickase Plasmid (h) and Op18 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STMN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Op18 Antibody (E-3): sc-55531
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Op18 Double Nickase Plasmid (h) | sc-401656-NIC | 20 µg | $410.00 | |||
Op18 Double Nickase Plasmid (h2) | sc-401656-NIC-2 | 20 µg | $410.00 |
STMN1 encodes stathmin/oncoprotein 18 (Op18), a cytosolic phosphoprotein that binds tubulin heterodimers and promotes microtubule catastrophe, thereby tuning spindle assembly, mitotic progression, and intracellular trafficking. Its activity is regulated by phosphorylation downstream of signaling nodes including MAPK and PI3K/AKT, coupling growth-factor inputs to cytoskeletal dynamics and cell-cycle control. Dysregulated STMN1 expression is frequently associated with proliferative and invasive phenotypes in cancer biology and has also been linked to altered neuronal microtubule stability in neurodegeneration models. As a result, STMN1 is widely studied in pathways governing mitosis, migration, and stress responses that reshape the microtubule network.
Op18 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STMN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STMN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STMN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STMN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.