Oligo (dT)15 Primer: sc-43502

The Oligo (dT)15 Primer is a pivotal reagent extensively utilized in scientific research, particularly in molecular biology and genomics studies. Its mechanism of action involves serving as a synthetic oligonucleotide primer with a sequence of 15 deoxythymidine residues, designed to anneal specifically to the polyadenylate (poly(A)) tails present at the 3′ end of eukaryotic mRNA molecules. In research applications, Oligo (dT)15 Primer serves as a crucial tool for reverse transcription reactions, where it selectively hybridizes to the poly(A) tails of mRNA transcripts, initiating the synthesis of complementary DNA (cDNA) strands by reverse transcriptase enzymes. Researchers exploit this primer to generate cDNA libraries from mRNA samples, enabling the analysis of gene expression profiles, transcriptomic studies, and gene discovery endeavors. Moreover, Oligo (dT)15 Primer facilitates the amplification of specific mRNA targets for downstream applications, such as quantitative real-time PCR (qPCR), gene expression analysis, and functional genomics assays. Its high specificity and efficiency in cDNA synthesis make it an indispensable component in molecular biology workflows, contributing to our understanding of gene regulation, signal transduction pathways, and cellular processes in scientific research.