OLFML3 CRISPR/Cas9 KO Plasmid (h): sc-404425
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OLFML3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OLFML3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OLFML3 CRISPR/Cas9 KO Plasmid (h) | sc-404425 | 20 µg | $397.00 |
Overview
OLFML3 (olfactomedin-like 3) encodes a secreted extracellular matrix–associated glycoprotein of the olfactomedin family implicated in cell–matrix interactions and tissue remodeling. It is expressed in stromal and vascular contexts and has been linked to regulation of angiogenic programs, fibroblast-like behavior, and modulation of inflammatory microenvironments through effects on extracellular signaling cues. OLFML3 activity intersects with pathways governing extracellular matrix organization, cell migration, and endothelial–stromal crosstalk, processes frequently altered during tumor progression and fibrotic remodeling. Dysregulated OLFML3 expression has been reported in multiple cancers and vascular-associated pathologies, supporting its utility as a mechanistic node for studying microenvironment-driven phenotypes.
OLFML3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OLFML3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OLFML3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OLFML3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OLFML3 protein expression.
This CRISPR knockout system enables efficient generation of OLFML3-deficient cell models for investigation of OLFML3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OLFML3 exon(s) critical for OLFML3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OLFML3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OLFML3 CRISPR/Cas9 KO Plasmid (h) and OLFML3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OLFML3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OLFML3 HDR Plasmid (h) and OLFML3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OLFML3 homology arms to support homology-directed repair at defined OLFML3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.