OGFRL1 CRISPR/Cas9 KO Plasmid (h): sc-407392
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OGFRL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OGFRL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OGFRL1 CRISPR/Cas9 KO Plasmid (h) | sc-407392 | 20 µg | $397.00 |
Overview
OGFRL1 (opioid growth factor receptor-like 1) encodes a receptor-like protein related to the opioid growth factor receptor family and is thought to participate in regulatory networks that link extracellular cues to intracellular control of cell growth and survival. Although the precise molecular mechanism remains incompletely defined, OGFRL1 has been implicated in pathways governing proliferation, differentiation, and tissue homeostasis, processes that are frequently remodeled during oncogenic transformation and microenvironmental adaptation. Altered OGFRL1 expression patterns have been reported in transcriptomic studies across multiple disease contexts, supporting its use as a candidate modulator in cell-state transitions. As a result, OGFRL1 is of interest for dissecting signaling crosstalk that influences growth control, stress responses, and cellular plasticity.
OGFRL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OGFRL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OGFRL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OGFRL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OGFRL1 protein expression.
This CRISPR knockout system enables efficient generation of OGFRL1-deficient cell models for investigation of OGFRL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OGFRL1 exon(s) critical for OGFRL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OGFRL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OGFRL1 CRISPR/Cas9 KO Plasmid (h) and OGFRL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OGFRL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OGFRL1 HDR Plasmid (h) and OGFRL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OGFRL1 homology arms to support homology-directed repair at defined OGFRL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.