Date published: 2026-4-5

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Oct3/4 Antibody (A-9): sc-365509

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Datasheets
  • Oct3/4 Antibody (A-9) is a mouse monoclonal IgG1 λ, cited in 21 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 16-45 near the N-terminus of Oct-3/4 of human origin
  • Anti-Oct3/4 Antibody (A-9) is recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
  • TransCruz reagent for ChIP application (sc-365509 X, 200 µg/0.1 ml)
  • Anti-Oct3/4 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Oct3/4 Antibody (A-9) for WB applications. This reagent is now offered in a bundle with Oct3/4 Antibody (A-9) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Oct-3/4 Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects amino acids 16-45 near the N-terminus of Oct-3/4 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Oct-3/4 monoclonal antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POU5F1, also known as Oct-3/4, plays a critical role in maintaining pluripotency and self-renewal of embryonic stem cells, which is essential for early development and tissue regeneration. Oct-3/4 (A-9) antibody targets this transcription factor in the nucleus, where Oct-3/4 binds to specific DNA sequences to regulate gene expression involved in stem cell maintenance and differentiation. Oct-3/4′s ability to modulate gene expression is vital for proper development of various tissues and organs, and dysregulation can lead to developmental disorders and cancer. In humans, two isoforms of Oct-3/4 influence the undifferentiated state of embryonic stem cells, while pseudogenes associated with Oct-3/4 are transcribed in certain cancer cell lines, highlighting Oct-3/4′s importance in both normal physiology and disease states. Oct-3/4 (A-9) monoclonal antibody provides a versatile tool for detecting and studying Oct-3/4′s critical functions in stem cell biology, development, and disease research.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Oct3/4 Antibody (A-9)

sc-365509
200 µg/ml
$322.00

Oct3/4 Antibody (A-9): m-IgGλ BP-HRP Bundle

sc-522262
200 µg Ab, 40 µg BP
$361.00

Oct3/4 Antibody (A-9) X

sc-365509 X
200 µg/0.1 ml
$322.00

Oct3/4 Antibody (A-9) AC

sc-365509 AC
500 µg/ml, 25% agarose
$424.00

Oct3/4 Antibody (A-9) HRP

sc-365509 HRP
200 µg/ml
$322.00

Oct3/4 Antibody (A-9) FITC

sc-365509 FITC
200 µg/ml
$336.00

Oct3/4 Antibody (A-9) PE

sc-365509 PE
200 µg/ml
$349.00

Oct3/4 Antibody (A-9) Alexa Fluor® 488

sc-365509 AF488
200 µg/ml
$364.00

Oct3/4 Antibody (A-9) Alexa Fluor® 546

sc-365509 AF546
200 µg/ml
$364.00

Oct3/4 Antibody (A-9) Alexa Fluor® 594

sc-365509 AF594
200 µg/ml
$364.00

Oct3/4 Antibody (A-9) Alexa Fluor® 647

sc-365509 AF647
200 µg/ml
$364.00

Oct3/4 Antibody (A-9) Alexa Fluor® 680

sc-365509 AF680
200 µg/ml
$364.00

Oct3/4 Antibody (A-9) Alexa Fluor® 790

sc-365509 AF790
200 µg/ml
$364.00

Oct3/4 (A-9) Neutralizing Peptide

sc-365509 P
100 µg/0.5 ml
$69.00

Does this antibody cross-react with OCT4B?

Asked by: timmy1027
Thank you for your question. Based on N-terminal antigenic sequence used to develop this mouse monoclonal antibody, we would not expect it to recognize OCT4B, also known as OCT4B-265, based on the published RefSeq protein sequence on NCBI for this isoform: NP_001272916.1 POU domain, class 5, transcription factor 1 isoform 3.
Answered by: Technical Support
Date published: 2025-03-05

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-20
  • y_2026, m_3, d_26, h_7CST
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Rated 5 out of 5 by from Great WB dataVery clean western blot data; strong signal, low background
Date published: 2017-02-05
Rated 5 out of 5 by from Looking for an Oct-3/4 antibody?Excellent choice for detecting Oct-3/4 in both mouse and human samples. Highly recommend this clone!
Date published: 2016-12-30
Rated 5 out of 5 by from The antibody works well for Western blottingThe antibody works well for Western blotting detecting endogenous Oct-3/4 from NTERA-2 cl.D1 whole cell lysate. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in F9 whole cell lysate. -SCBT QC
Date published: 2013-06-03
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Oct3/4 Antibody (A-9) is rated 5.0 out of 5 by 4.
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