OC-STAMP CRISPR/Cas9 KO Plasmid (h): sc-406422
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OC-STAMP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OC-STAMP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OC-STAMP CRISPR/Cas9 KO Plasmid (h) | sc-406422 | 20 µg | $397.00 |
Overview
OCSTAMP encodes OC‑STAMP, a multi-pass transmembrane protein required for osteoclast cell–cell fusion and the formation of multinucleated, bone-resorbing osteoclasts. OC‑STAMP cooperates with fusion regulators such as DCSTAMP and integrates with RANKL-driven osteoclastogenesis programs, influencing cytoskeletal remodeling and membrane dynamics during differentiation. Through its role in osteoclast maturation, altered OCSTAMP activity is relevant to studies of skeletal homeostasis and disorders characterized by dysregulated bone resorption, including osteolytic pathology in inflammatory and metabolic contexts. In addition, OC‑STAMP expression in myeloid-lineage cells supports investigation of how fusion competence impacts immune–bone crosstalk and tissue remodeling.
OC-STAMP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OCSTAMP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OCSTAMP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OCSTAMP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OC-STAMP protein expression.
This CRISPR knockout system enables efficient generation of OCSTAMP-deficient cell models for investigation of OC-STAMP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OCSTAMP exon(s) critical for OC-STAMP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OCSTAMP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OC-STAMP CRISPR/Cas9 KO Plasmid (h) and OC-STAMP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OCSTAMP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OC-STAMP HDR Plasmid (h) and OC-STAMP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OCSTAMP homology arms to support homology-directed repair at defined OCSTAMP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.