OC-3 CRISPR/Cas9 KO Plasmid (h): sc-406357
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OC-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OC-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OC-3 CRISPR/Cas9 KO Plasmid (h) | sc-406357 | 20 µg | $397.00 |
Overview
ONECUT3 encodes the transcription factor OC-3, a member of the onecut family characterized by a CUT domain and homeobox DNA-binding domain that coordinate tissue-specific gene expression programs. OC-3 contributes to regulation of cellular differentiation and lineage specification by controlling transcriptional networks that shape developmental and metabolic states. In human biology, ONECUT3 expression patterns have been linked to modulation of epithelial and hepatic gene regulatory circuitry and broader transcriptional control of organogenesis-related pathways. Dysregulated onecut-family activity has been examined in the context of altered differentiation, proliferation, and tumor-associated transcriptional reprogramming, supporting its use as a node for mechanistic studies of disease-relevant gene networks.
OC-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ONECUT3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ONECUT3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ONECUT3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OC-3 protein expression.
This CRISPR knockout system enables efficient generation of ONECUT3-deficient cell models for investigation of OC-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ONECUT3 exon(s) critical for OC-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ONECUT3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OC-3 CRISPR/Cas9 KO Plasmid (h) and OC-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ONECUT3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OC-3 HDR Plasmid (h) and OC-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ONECUT3 homology arms to support homology-directed repair at defined ONECUT3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.