OATP-H CRISPR/Cas9 KO Plasmid (h): sc-404706
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OATP-H CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OATP-H genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OATP-H CRISPR/Cas9 KO Plasmid (h) | sc-404706 | 20 µg | $397.00 |
Overview
SLCO4C1 encodes the human organic anion transporting polypeptide OATP-H (OATP4C1), a multispecific solute carrier that mediates sodium-independent uptake of a range of endogenous metabolites and xenobiotic-like organic anions across cellular membranes. This transporter contributes to transmembrane transport processes that shape cellular exposure to circulating compounds and influence downstream metabolic handling and detoxification pathways. Altered expression or activity of OATP family members has been associated with changes in tissue distribution of substrates and perturbations in metabolic homeostasis, making SLCO4C1 a useful target for studying transport-driven phenotypes. In biomedical research, SLCO4C1 perturbation supports mechanistic interrogation of transporter–substrate relationships and their impact on cell signaling and stress responses.
OATP-H CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLCO4C1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLCO4C1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLCO4C1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OATP-H protein expression.
This CRISPR knockout system enables efficient generation of SLCO4C1-deficient cell models for investigation of OATP-H signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLCO4C1 exon(s) critical for OATP-H function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLCO4C1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OATP-H CRISPR/Cas9 KO Plasmid (h) and OATP-H CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLCO4C1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OATP-H HDR Plasmid (h) and OATP-H HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLCO4C1 homology arms to support homology-directed repair at defined SLCO4C1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.