Oasl1 CRISPR/Cas9 KO Plasmid (m): sc-433090
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Oasl1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Oasl1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oasl1 CRISPR/Cas9 KO Plasmid (m) | sc-433090 | 20 µg | $397.00 |
Overview
Oasl1 (2′-5′-oligoadenylate synthetase-like 1) is a mouse interferon-stimulated gene implicated in innate immune regulation during antiviral responses. Unlike catalytically active OAS family members, Oasl1 primarily functions through protein–protein and RNA-associated interactions that modulate type I interferon signaling and downstream transcriptional programs. It is linked to control of interferon regulatory factor pathways and broader inflammatory circuitry that shapes immune cell activation and cytokine output. Dysregulated Oasl1 activity is therefore relevant to studying mechanisms of aberrant interferon responses and immune-mediated pathology in infection and inflammatory disease models.
Oasl1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Oasl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Oasl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Oasl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Oasl1 protein expression.
This CRISPR knockout system enables efficient generation of Oasl1-deficient cell models for investigation of Oasl1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Oasl1 exon(s) critical for Oasl1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Oasl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Oasl1 CRISPR/Cas9 KO Plasmid (m) and Oasl1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Oasl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Oasl1 HDR Plasmid (m) and Oasl1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Oasl1 homology arms to support homology-directed repair at defined Oasl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.