O6-Benzylguanine (CAS 19916-73-5)

O6-Benzylguanine (O6-BG) is a highly versatile organobenzylguanine derivative extensively utilized in organic synthesis, biochemistry, and molecular biology. This compound serves multiple purposes, including guanine derivative synthesis, DNA methyltransferase inhibition, and protein-protein interaction studies. As a potent and selective inhibitor, O6-Benzylguanine targets DNA methyltransferases (DNMTs), enzymes involved in epigenetic gene expression regulation. Furthermore, O6-Benzylguanine facilitates the investigation of protein-protein interactions by binding to proteins and inducing conformational changes. It finds utility in in vitro and in vivo experiments to explore the impact of epigenetic regulation on gene expression. Although the precise mechanism of action of O6-Benzylguanine remains incompletely understood, it is believed to hinder DNMTs by binding to their active sites and impeding the transfer of methyl groups, thereby inhibiting enzyme activity. Notably, O6-Benzylguanine exhibits diverse biochemical and physiological effects. It inhibits DNMT activity, leading to alterations in gene expression. Additionally, O6-Benzylguanine influences the activity of various proteins, influencing metabolic pathways, as well as the production of proteins and hormones. Moreover, O6-Benzylguanine has demonstrated anti-inflammatory, anti-cancer, and anti-viral properties in vitro.