O-GlcNAc transferase Antibody (F-12): sc-74546
- O-GlcNAc transferase Antibody (F-12) is a mouse monoclonal IgG2a κ O-GlcNAc transferase antibody, cited in 36 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of O-GlcNAc transferase of human origin
- O-GlcNAc transferase Antibody (F-12) is recommended for detection of O-GlcNAc transferase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-O-GlcNAc transferase Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for O-GlcNAc transferase Antibody (F-12) for WB and IHC(P) applications. These reagents are now offered in bundles with O-GlcNAc transferase Antibody (F-12) (see ordering information below).
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O-GlcNAc transferase Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects O-GlcNAc transferase of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). O-GlcNAc transferase (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. O-linked N-acetylglucosamine transferase, also known as OGT, plays a crucial role in cellular signaling by catalyzing the addition of a single N-acetylglucosamine moiety to serine or threonine residues on target proteins. This post-translational modification influences protein function, stability, and interactions, thereby impacting various cellular processes such as transcription, cell cycle regulation, and stress responses. The dynamic interplay between O-GlcNAcylation and phosphorylation at these residues is particularly important, as they can compete for the same sites, leading to functional outcomes that are critical for maintaining cellular homeostasis. O-GlcNAc transferase is expressed in multiple human tissues, with the pancreas showing the highest levels, and exists as a heterotrimeric complex, indicating potential involvement in complex regulatory networks within the cell. Immunofluorescence (IF) studies have shown that O-GlcNAc transferase localizes in both the nucleus and cytosol, suggesting a multifaceted role in cellular functions. Notably, prolonged expression of O-GlcNAc transferase in HeLa cells has been associated with reduced cell viability, highlighting potential toxicity and the need for careful consideration in experimental designs.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
O-GlcNAc transferase Antibody (F-12) References:
- The O-GlcNAc transferase gene resides on the X chromosome and is essential for embryonic stem cell viability and mouse ontogeny. | Shafi, R., et al. 2000. Proc Natl Acad Sci U S A. 97: 5735-9. PMID: 10801981
- Localization of the O-GlcNAc transferase and O-GlcNAc-modified proteins in rat cerebellar cortex. | Akimoto, Y., et al. 2003. Brain Res. 966: 194-205. PMID: 12618343
- Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase. | Haltiwanger, RS., et al. 1992. J Biol Chem. 267: 9005-13. PMID: 1533623
- Identification of secret agent as the O-GlcNAc transferase that participates in Plum pox virus infection. | Chen, D., et al. 2005. J Virol. 79: 9381-7. PMID: 16014901
- A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates. | Leavy, TM. and Bertozzi, CR. 2007. Bioorg Med Chem Lett. 17: 3851-4. PMID: 17531489
- Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance. | Yang, X., et al. 2008. Nature. 451: 964-9. PMID: 18288188
- Advances in chemical probing of protein O-GlcNAc glycosylation: structural role and molecular mechanisms. | Saha, A., et al. 2021. Chem Soc Rev. 50: 10451-10485. PMID: 34338261
- O-GlcNAc transferase regulates intervertebral disc degeneration by targeting FAM134B-mediated ER-phagy. | Luo, R., et al. 2022. Exp Mol Med. 54: 1472-1485. PMID: 36056188
- O-GlcNAc transferase in astrocytes modulates depression-related stress susceptibility through glutamatergic synaptic transmission. | Fan, J., et al. 2023. J Clin Invest. 133: PMID: 36757814
- Hepatocyte-specific O-GlcNAc transferase downregulation ameliorates nonalcoholic steatohepatitis by improving mitochondrial function. | Gonzalez-Rellan, MJ., et al. 2023. Mol Metab. 75: 101776. PMID: 37453647
- Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats. | Kreppel, LK., et al. 1997. J Biol Chem. 272: 9308-15. PMID: 9083067
- O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats. | Lubas, WA., et al. 1997. J Biol Chem. 272: 9316-24. PMID: 9083068
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
O-GlcNAc transferase Antibody (F-12) | sc-74546 | 200 µg/ml | $322.00 | |||
O-GlcNAc transferase Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-525728 | 200 µg Ab; 10 µg BP | $361.00 | |||
O-GlcNAc transferase Antibody (F-12): m-IgG2a BP-HRP Bundle | sc-546384 | 200 µg Ab; 10 µg BP | $361.00 | |||
O-GlcNAc transferase Antibody (F-12) AC | sc-74546 AC | 500 µg/ml, 25% agarose | $424.00 | |||
O-GlcNAc transferase Antibody (F-12) HRP | sc-74546 HRP | 200 µg/ml | $322.00 | |||
O-GlcNAc transferase Antibody (F-12) FITC | sc-74546 FITC | 200 µg/ml | $336.00 | |||
O-GlcNAc transferase Antibody (F-12) PE | sc-74546 PE | 200 µg/ml | $349.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 488 | sc-74546 AF488 | 200 µg/ml | $364.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 546 | sc-74546 AF546 | 200 µg/ml | $364.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 594 | sc-74546 AF594 | 200 µg/ml | $364.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 647 | sc-74546 AF647 | 200 µg/ml | $364.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 680 | sc-74546 AF680 | 200 µg/ml | $364.00 | |||
O-GlcNAc transferase Antibody (F-12) Alexa Fluor® 790 | sc-74546 AF790 | 200 µg/ml | $364.00 |