NYNRIN CRISPR/Cas9 KO Plasmid (h): sc-410066
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NYNRIN CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NYNRIN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NYNRIN CRISPR/Cas9 KO Plasmid (h) | sc-410066 | 20 µg | $397.00 |
Overview
NYNRIN encodes a large multidomain protein reported to contain NYN and retroviral integrase–like regions, suggesting links to nucleic acid or protein complex interactions, although its precise molecular function remains incompletely characterized in human cells. Transcriptomic studies have associated NYNRIN expression with developmental and differentiation programs and with regulation of cellular homeostasis in specific tissues. Altered NYNRIN expression has been observed across multiple disease contexts, supporting its use as a candidate factor for investigating dysregulated gene expression networks. As a relatively understudied gene, NYNRIN is often explored to define pathway connectivity, cell-state transitions, and phenotypes emerging from perturbation of poorly annotated regulators.
NYNRIN CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NYNRIN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NYNRIN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NYNRIN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NYNRIN protein expression.
This CRISPR knockout system enables efficient generation of NYNRIN-deficient cell models for investigation of NYNRIN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NYNRIN exon(s) critical for NYNRIN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NYNRIN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NYNRIN CRISPR/Cas9 KO Plasmid (h) and NYNRIN CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NYNRIN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NYNRIN HDR Plasmid (h) and NYNRIN HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NYNRIN homology arms to support homology-directed repair at defined NYNRIN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.