Nur77 (m): 293T Lysate: sc-122180

Nur77 (m): 293T Lysate, derived from the HEK 293T cell line, is essential for studying the orphan nuclear receptor Nur77 (NR4A1) in murine systems. HEK 293T cells, a variant of the HEK 293 cells, are engineered to express the SV40 large T antigen, significantly enhancing their protein production capabilities. This lysate is particularly valuable for investigating the role of Nur77 in regulating gene expression, apoptosis, and metabolic processes. Researchers utilize Nur77 (m): 293T Lysate to explore the mechanisms by which Nur77 influences various cellular pathways. Nur77 can translocate from the nucleus to the mitochondria in response to certain stimuli, where it interacts with Bcl-2, converting it from a protector to a promoter of apoptosis. Techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and luciferase reporter assays are commonly employed to study Nur77's DNA binding activity and its role in transcriptional regulation. This lysate helps explain Nur77's involvement in cellular stress responses and metabolic regulation. By analyzing Nur77's interactions and regulatory mechanisms, scientists can better understand its contribution to fundamental biological processes. Research using Nur77 (m): 293T Lysate is strictly focused on basic scientific inquiry, thereby deepening our understanding of cellular regulation and signaling pathways.