Nur77 Double Nickase Plasmid (h): sc-418320-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nur77 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nur77 Double Nickase Plasmid (h) and Nur77 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR4A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nur77 Antibody (C-5): sc-365113
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nur77 Double Nickase Plasmid (h) | sc-418320-NIC | 20 µg | $410.00 | |||
Nur77 Double Nickase Plasmid (h2) | sc-418320-NIC-2 | 20 µg | $410.00 |
NR4A1 (Nur77) is an orphan nuclear receptor and immediate-early transcription factor that integrates mitogenic, inflammatory, and stress cues to rapidly reprogram gene expression. It functions in nuclear receptor signaling to regulate apoptosis, proliferation, metabolism, and immune cell differentiation, with context-dependent effects in T cell activation/tolerance, macrophage polarization, and vascular biology. Nur77 activity intersects with MAPK, NF-κB, and PI3K/AKT-linked transcriptional networks, shaping cytokine programs and cellular stress responses. Dysregulated NR4A1 expression or signaling has been associated with inflammatory disorders, atherosclerosis, and diverse cancer-relevant phenotypes, making it a useful node for mechanistic studies of transcriptional control and cell fate decisions.
Nur77 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR4A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR4A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR4A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR4A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.