Nup50 Antibody (G-4): sc-398993
- Nup50 Antibody (G-4) is a mouse monoclonal IgG1 λ Nup50 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 43-166 mapping near the N-terminus of Nup50 of human origin
- Nup50 Antibody (G-4) is recommended for detection of Nup50 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Nup50 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Nup50 Antibody (G-4) for WB applications. This reagent is now offered in a bundle with Nup50 Antibody (G-4) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Nup50 Antibody (G-4) is a mouse monoclonal IgG1 antibody that detects Nup50 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nup50 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nup50, also known as nucleoporin 50 or NPAP60, is a crucial component of nuclear pore complexes (NPCs), which facilitate the transport of macromolecules between the nucleus and cytoplasm. This 468 amino acid protein plays a vital role as a binding site for export receptor-cargo complexes, localizing to the nucleoplasmic fibrils of the NPC. Nup50′s interaction with various transport receptor proteins, including p27, underscores its importance in cellular transport mechanisms. The presence of multiple phenylalanine-glycine (FG) repeats and a RanBD1 domain in Nup50 contributes to its functionality and interaction with other nucleoporins, which are essential for maintaining the integrity and efficiency of nucleocytoplasmic transport. While Nup50 is ubiquitously expressed, Nup50 is particularly abundant in peripheral blood leukocytes, testis, and fetal liver, highlighting its significance in both normal physiology and potential pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nup50 Antibody (G-4) References:
- Mapping and complex expression pattern of the human NPAP60L nucleoporin gene. | Trichet, V., et al. 1999. Cytogenet Cell Genet. 85: 221-6. PMID: 10449902
- Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export. | Guan, T., et al. 2000. Mol Cell Biol. 20: 5619-30. PMID: 10891499
- Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex. | Smitherman, M., et al. 2000. Mol Cell Biol. 20: 5631-42. PMID: 10891500
- Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import. | Lindsay, ME., et al. 2002. Cell. 110: 349-60. PMID: 12176322
- Nucleocytoplasmic transport: more than the usual suspects. | Swaminathan, S. and Melchior, F. 2002. Dev Cell. 3: 304-6. PMID: 12361592
- Nucleophosmin is essential for ribosomal protein L5 nuclear export. | Yu, Y., et al. 2006. Mol Cell Biol. 26: 3798-809. PMID: 16648475
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup50 Antibody (G-4) | sc-398993 | 200 µg/ml | $316.00 | |||
Nup50 Antibody (G-4): m-IgGλ BP-HRP Bundle | sc-524568 | 200 µg Ab, 40 µg BP | $354.00 | |||
Nup50 Antibody (G-4) AC | sc-398993 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Nup50 Antibody (G-4) HRP | sc-398993 HRP | 200 µg/ml | $316.00 | |||
Nup50 Antibody (G-4) FITC | sc-398993 FITC | 200 µg/ml | $330.00 | |||
Nup50 Antibody (G-4) PE | sc-398993 PE | 200 µg/ml | $343.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 488 | sc-398993 AF488 | 200 µg/ml | $357.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 546 | sc-398993 AF546 | 200 µg/ml | $357.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 594 | sc-398993 AF594 | 200 µg/ml | $357.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 647 | sc-398993 AF647 | 200 µg/ml | $357.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 680 | sc-398993 AF680 | 200 µg/ml | $357.00 | |||
Nup50 Antibody (G-4) Alexa Fluor® 790 | sc-398993 AF790 | 200 µg/ml | $357.00 |