Nup37 CRISPR/Cas9 KO Plasmid (h): sc-409458
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nup37 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nup37 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup37 CRISPR/Cas9 KO Plasmid (h) | sc-409458 | 20 µg | $397.00 |
Overview
NUP37 encodes Nup37, an essential component of the Nup107–160 subcomplex of the nuclear pore complex that supports nuclear envelope integrity and selective nucleocytoplasmic transport. By contributing to nuclear pore assembly and gating, Nup37 influences RNA export, protein import, and the spatial regulation of transcriptional programs that depend on proper nuclear trafficking. Disruption of nuclear pore composition can perturb genome stability and mitotic progression, processes frequently altered in proliferative disease contexts. As a result, NUP37 is commonly studied in pathways linking nuclear transport, cell-cycle control, and stress-responsive signaling.
Nup37 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NUP37 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NUP37 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NUP37 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nup37 protein expression.
This CRISPR knockout system enables efficient generation of NUP37-deficient cell models for investigation of Nup37 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NUP37 exon(s) critical for Nup37 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NUP37 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nup37 CRISPR/Cas9 KO Plasmid (h) and Nup37 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NUP37 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nup37 HDR Plasmid (h) and Nup37 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NUP37 homology arms to support homology-directed repair at defined NUP37 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.