Nup205 Antibody (H-1): sc-377047
- Nup205 Antibody (H-1) is a mouse monoclonal IgG1 κ Nup205 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 661-960 mapping within an internal region of Nup205 of human origin
- Nup205 Antibody (H-1) is recommended for detection of Nup205 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Nup205 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Nup205 Antibody (H-1) for WB applications. This reagent is now offered in a bundle with Nup205 Antibody (H-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Nup205 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nup205 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nup205 antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nup205, also known as nucleoporin 205 kDa, is a critical component of the nuclear pore complex (NPC), which facilitates the transport of macromolecules between the nucleus and cytoplasm in eukaryotic cells. Nup205 is composed of 2,012 amino acids and is characterized by the presence of O-linked-N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG), which are essential for structural integrity and function. Nup205′s localization to the nucleus is vital for maintaining proper NPC functioning, as Nup205 plays a key role in regulating nucleocytoplasmic transport, a process crucial for cellular homeostasis and gene expression. The gene encoding Nup205 is located on human chromosome 7, a region associated with various genetic disorders, highlighting Nup205′s importance in cellular processes and potential implications in disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nup205 Antibody (H-1) References:
- Docking of HIV-1 Vpr to the nuclear envelope is mediated by the interaction with the nucleoporin hCG1. | Le Rouzic, E., et al. 2002. J Biol Chem. 277: 45091-8. PMID: 12228227
- Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex. | Hase, ME. and Cordes, VC. 2003. Mol Biol Cell. 14: 1923-40. PMID: 12802065
- Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo. | Galy, V., et al. 2003. Mol Biol Cell. 14: 5104-15. PMID: 12937276
- Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex. | Hawryluk-Gara, LA., et al. 2005. Mol Biol Cell. 16: 2382-94. PMID: 15703211
- The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells. | Mansfeld, J., et al. 2006. Mol Cell. 22: 93-103. PMID: 16600873
- Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly. | Ródenas, E., et al. 2009. Dev Biol. 327: 399-409. PMID: 19146848
- Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. | Grandi, P., et al. 1997. Mol Biol Cell. 8: 2017-38. PMID: 9348540
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup205 Antibody (H-1) | sc-377047 | 200 µg/ml | $316.00 | |||
Nup205 Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-523168 | 200 µg Ab, 40 µg BP | $354.00 | |||
Nup205 Antibody (H-1) AC | sc-377047 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Nup205 Antibody (H-1) HRP | sc-377047 HRP | 200 µg/ml | $316.00 | |||
Nup205 Antibody (H-1) FITC | sc-377047 FITC | 200 µg/ml | $330.00 | |||
Nup205 Antibody (H-1) PE | sc-377047 PE | 200 µg/ml | $343.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 488 | sc-377047 AF488 | 200 µg/ml | $357.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 546 | sc-377047 AF546 | 200 µg/ml | $357.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 594 | sc-377047 AF594 | 200 µg/ml | $357.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 647 | sc-377047 AF647 | 200 µg/ml | $357.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 680 | sc-377047 AF680 | 200 µg/ml | $357.00 | |||
Nup205 Antibody (H-1) Alexa Fluor® 790 | sc-377047 AF790 | 200 µg/ml | $357.00 |