Nup153 (m): 293T Lysate: sc-122173

Nup153 (m): 293T Lysate, derived from the HEK 293T cell line, is a valuable resource for studying the nuclear pore complex protein Nup153 in murine models. HEK 293T cells, a variant of the original HEK 293 cells, are modified to express the SV40 large T antigen, enhancing their ability to produce high levels of recombinant proteins. This lysate is particularly useful for examining the role of Nup153 in nuclear transport and the structural integrity of the nuclear pore complex (NPC). Researchers use Nup153 (m): 293T Lysate to investigate the mechanisms by which Nup153 contributes to the selective transport of molecules between the nucleus and cytoplasm. Nup153 is known for its involvement in anchoring other nucleoporins to the nuclear basket, a crucial part of the NPC, and facilitating the transport of RNA and ribonucleoproteins. Techniques such as immunoprecipitation, Western blotting, and fluorescence microscopy are employed to study the interactions of Nup153 with other NPC components and its role in regulating nucleocytoplasmic transport. This lysate is instrumental in elucidating the molecular dynamics of NPC assembly and function, providing insights into the fundamental processes of nuclear transport regulation. Research using Nup153 (m): 293T Lysate focuses strictly on basic scientific inquiry, enhancing our understanding of cellular compartmentalization and transport mechanisms.