Nup153 Antibody (R4C8): sc-101545

Nup153 Antibody (R4C8) is a rat monoclonal IgG2a antibody that detects Nup153 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nup153 plays a crucial role in the assembly and function of nuclear pore complexes (NPCs), which are essential for the regulated transport of macromolecules between the nucleus and cytoplasm. Nup153 is strategically located at the periphery of NPCs, where anti-Nup153 antibody (R4C8) can detect its interaction between the nuclear pore and the nuclear lamina, thereby influencing the structural integrity of the nuclear envelope. The unique structural features of Nup153, including its N-terminal region, central domain with multiple zinc fingers, and a C-terminal region rich in FXFG repeats, are vital for its function in mediating specific nuclear import events and anchoring NPCs within the nuclear envelope. Nup153 is cleaved by caspases during apoptosis, highlighting its involvement in cellular processes beyond transport, such as programmed cell death. Nup153′s ability to interact with proteins like TAP, which is critical for mRNA export, underscores its importance in gene expression regulation and cellular homeostasis. With diverse applications and significant role in cellular transport mechanisms, Nup153 monoclonal antibody (R4C8) serves as an invaluable tool for researchers studying nuclear dynamics and related pathways.

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Nup153 Antibody (R4C8) References:

1. Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain.  |  Nakielny, S., et al. 1999. EMBO J. 18: 1982-95. PMID: 10202161
2. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.  |  Walther, TC., et al. 2001. EMBO J. 20: 5703-14. PMID: 11598013
3. The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells.  |  Zhou, L. and Panté, N. 2010. FEBS Lett. 584: 3013-20. PMID: 20561986
4. The Nup153-Nup50 protein interface and its role in nuclear import.  |  Makise, M., et al. 2012. J Biol Chem. 287: 38515-22. PMID: 23007389
5. Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy.  |  Duheron, V., et al. 2014. Nucleus. 5: 601-12. PMID: 25485891
6. The nuclear pore protein Nup153 associates with chromatin and regulates cardiac gene expression in dystrophic mdx hearts.  |  Nanni, S., et al. 2016. Cardiovasc Res. 112: 555-567. PMID: 28513807
7. Nup153 and Nup50 promote recruitment of 53BP1 to DNA repair foci by antagonizing BRCA1-dependent events.  |  Mackay, DR., et al. 2017. J Cell Sci. 130: 3347-3359. PMID: 28751496
8. Nup153 Unlocks the Nuclear Pore Complex for HIV-1 Nuclear Translocation in Nondividing Cells.  |  Buffone, C., et al. 2018. J Virol. 92: PMID: 29997211
9. Expression of the NUP153 and YWHAB genes from their canonical promoters and alternative promoters of the LINE-1 retrotransposon in the placenta of the first trimester of pregnancy.  |  Demeneva, VV., et al. 2023. Vavilovskii Zhurnal Genet Selektsii. 27: 63-71. PMID: 36923475
10. Novel Function of NUP153 in HNF4α Transcriptional Upregulation Contributes to Promoting HBV Replication.  |  Jiang, Q., et al. 2025. J Med Virol. 97: e70256. PMID: 40014546

The preferred Nup153 antibody is Nup153 Antibody (R3G1): sc-101544.